Deposition of Cellulose Nanocrystals onto Supported Lipid Membranes

The deposition of cellulose nanocrystals (CNCs) on a supported lipid bilayer (SLB) was investigated at different length scales. Quartz crystal microbalance with dissipation monitoring (QCM-D) was used to probe the bilayer formation and to show for the first time the CNC deposition onto the SLB. Specifically, classical QCM-D measurements gave estimation of the adsorbed hydrated mass and the corresponding film thickness, whereas complementary experiments using D2O as the solvent allowed the quantitative determination of the hydration of the CNC layer, showing a high hydration value. Scanning force microscopy (SFM) and total internal reflection fluorescence microscopy (TIRF) were used to probe the homogeneity of the deposited layers, revealing the structural details at the particle and film length scales, respectively, thus giving information on the effect of CNC concentration on the surface coverage. The results showed that the adsorption of CNCs on the supported lipid membrane depended on lipid composition, CNC concentration, and pH conditions, and that the binding process was governed by electrostatic interactions. Under suitable conditions, a uniform film was formed, with thickness corresponding to a CNC monolayer, which provides the basis for a relevant 2D model of a primary plant cell wall.