Sequential One-Pot Three-Enzyme Synthesis of the Tetrasaccharide Repeating Unit of Group B Streptococcus Serotype VIII Capsular Polysaccharide

被引：4
作者：
Liang, Min [1 ,3 ]
Gong, Wei [1 ,2 ]
Sun, Chongzhen [1 ,3 ]
Zhao, Jielin [1 ,3 ]
Wang, Hong [1 ,3 ]
Chen, Zonggang [1 ,3 ]
Xiao, Min [1 ,3 ]
Gu, Guofeng [1 ,3 ]
机构：
[1] Shandong Univ, Shandong Prov Key Lab Carbohydrate Chem & Glycobi, Natl Glycoengn Res Ctr, 72 Binhai Rd, Qingdao 266237, Shandong, Peoples R China
[2] Shandong Univ, Sch Pharmaceut Sci, 44 West Wenhua Rd, Jinan 250012, Shandong, Peoples R China
[3] Shandong Univ, NMPA Key Lab Qual Res & Evaluat Carbohydrate Base, Qingdao 266237, Shandong, Peoples R China
来源：
CHINESE JOURNAL OF CHEMISTRY | 2022年 / 40卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Streptococcus agalactiae; Capsular polysaccharide; Oligosaccharides; Enzyme catalysis; Biosynthesis; INFECTIONS; BIOSYNTHESIS; PNEUMONIAE; VACCINE;
D O I：
10.1002/cjoc.202100822
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Comprehensive Summary The biochemical property and functional identification of three recombinant glycosyltransferases, including beta-1,4-rhamnosyltransferase (Cps8R), beta-1,4-galactosyltransferase (Cps8J) and alpha-2,3-sialyltransferase (Cps8K) involved in the biosynthesis of the tetrasaccharide repeating unit of serotype VIII capsular polysaccharide (CPS) of Group B Streptococci (GBS), were systematically investigated. Subsequently, these recombinant enzymes were employed for one-pot three-enzyme efficient synthesis of the tetrasaccharide repeating unit of GBS serotype VIII CPS using the chemically synthesized Glc alpha-PP-(CH2)(11)-OPh as the starting substrate in a satisfying yield of 87%.
引用
收藏
页码：1039 / 1044
页数：6
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