Application of high-performance liquid chromatography with isotope-ratio mass spectrometry for measuring low levels of enrichment of underivatized materials

被引：15

作者：

Abramson, FP ^{[1
]}

Black, GE ^{[1
]}

Lecchi, P ^{[1
]}

机构：

[1] George Washington Univ, Sch Med & Hlth Sci, Dept Pharmacol, Washington, DC 20037 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 913卷 / 1-2期

关键词：

isotope-ratio mass spectrometry; mass spectrometry; proteins; carbohydrates; nucleotides;

D O I：

10.1016/S0021-9673(00)01032-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Combining HPLC separations with an isotope-ratio mass spectrometric (IRMS) detection produces a device capable of measuring very low alterations in C-13 abundance from analyte species that cannot be volatilized. Examples are presented showing proteins, carbohydrates, and nucleotides that are eluted from varying types of HPLC columns (reversed-phase, normal-phase, ion-exchange and size-exclusion). This wide range of chromatographic methods enables the analysis of compounds never before amenable to IRMS techniques and may lead to the development of many new assays. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：269 / 273

页数：5

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