The antioxidant Rutin counteracts the pathological impact of α-synuclein on the enteric nervous system in vitro

被引:6
作者
Christmann, Anne [1 ]
Gries, Manuela [1 ]
Scholz, Patrik [3 ]
Stahr, Pascal L. [4 ]
Law, Jessica Ka Yan [1 ]
Schulte, Steven [1 ]
Martin, Monika [1 ]
Lilischkis, Rainer [1 ]
Ingebrandt, Sven [5 ]
Keck, Cornelia M. [4 ]
Schaefer, Karl-Herbert [1 ,2 ]
机构
[1] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst & Technol, Working Grp Enter Nervous Syst, D-66482 Zweibrucken, Germany
[2] Heidelberg Univ, Med Fac Mannheim, Dept Pediat Surg, D-68167 Mannheim, Germany
[3] Bayer AG, R&D, Formulat Dev, D-51373 Leverkusen, Germany
[4] Philipps Univ Marburg, Dept Pharmaceut & Biopharmaceut, D-35037 Marburg, Germany
[5] Rhein Westfal TH Aachen, Inst Mat Elect Engn, D-52074 Aachen, Germany
关键词
A53T-alpha-synuclein; enteric nervous system; natural antioxidants; oxidative stress; Parkinson's disease; Rutin nanocrystals; PROTECTS DOPAMINERGIC-NEURONS; PARKINSONS-DISEASE; OXIDATIVE STRESS; REACTIVE OXYGEN; MITOCHONDRIAL DYSFUNCTION; RAT MODEL; BRAIN; DAMAGE; INFLAMMATION; NEUROINFLAMMATION;
D O I
10.1515/hsz-2021-0259
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Motoric disturbances in Parkinson's disease (PD) derive from the loss of dopaminergic neurons in the substantia nigra. Intestinal dysfunctions often appear long before manifestation of neuronal symptoms, suggesting a strong correlation between gut and brain in PD. Oxidative stress is a key player in neurodegeneration causing neuronal cell death. Using natural antioxidative flavonoids like Rutin, might provide intervening strategies to improve PD pathogenesis. To explore the potential effects of micro (mRutin) compared to nano Rutin (nRutin) upon the brain and the gut during PD, its neuroprotective effects were assessed using an in vitro PD model. Our results demonstrated that Rutin inhibited the neurotoxicity induced by A53T alpha-synuclein (Syn) administration by decreasing oxidized lipids and increasing cell viability in both, mesencephalic and enteric cells. For enteric cells, neurite outgrowth, number of synaptic vesicles, and tyrosine hydroxylase positive cells were significantly reduced when treated with Syn. This could be reversed by the addition of Rutin. nRutin revealed a more pronounced result in all experiments. In conclusion, our study shows that Rutin, especially the nanocrystals, are promising natural compounds to protect neurons from cell death and oxidative stress during PD. Early intake of Rutin may provide a realizable option to prevent or slow PD pathogenesis.
引用
收藏
页码:103 / 122
页数:20
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