Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

被引：51

作者：

Altamura, Gennaro ^{[1
]}

Corteggio, Annunziata ^{[1
]}

Pacini, Laura ^{[2
]}

Conte, Andrea ^{[3
]}

Pierantoni, Giovanna Maria ^{[3
]}

Tommasino, Massimo ^{[2
]}

Accardi, Rosita ^{[2
]}

Borzacchiello, Giuseppe ^{[1
]}

机构：

[1] Univ Naples Federico II, Gen Pathol & Pathol Anat Unit, Dept Vet Med & Anim Prod, Via Delpino 1, I-80137 Naples, Italy

[2] Int Agcy Res Canc, Infect & Canc Biol Grp, 150 Cours Albert Thomas, F-69372 Lyon, France

[3] Univ Naples Federico II, Dept Mol Med & Med Biotechnol, Via Pansini 5, I-80131 Naples, Italy

来源：

VIROLOGY | 2016年 / 496卷

关键词：

Felis catus papillomavirus; Feline SCC; Veterinary medicine; Feline oncology; E6; E7; Veterinary virology; Oncogenic viruses; LARYNGEAL-CANCER CELLS; RETINOBLASTOMA PROTEIN; INDUCED APOPTOSIS; GENE-EXPRESSION; DOWN-REGULATION; CUTANEOUS HPV; P53; DEGRADATION; ONCOPROTEINS; INHIBITION;

D O I：

10.1016/j.virol.2016.05.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with DV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：1 / 8

页数：8

共 45 条

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