Two electrical potential-dependent steps are required for transport by the Escherichia coli Tat machinery

被引:61
作者
Bageshwar, Umesh K. [1 ]
Musser, Siegfried M. [1 ]
机构
[1] Texas A&M Univ, Ctr Hlth Sci, Coll Med, Dept Mol & Cellular Med, College Stn, TX 77843 USA
关键词
D O I
10.1083/jcb.200702082
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The twin-arginine translocation ( Tat) pathway in Escherichia coli transports fully folded and assembled proteins across the energy-transducing periplasmic membrane. In chloroplasts, Tat transport requires energy input only from the proton motive force. To elucidate the mechanism and energetics of bacterial Tat protein transport, we developed an efficient in vitro transport assay using TatABC-enriched inverted membrane vesicles and the physiological precursor pre-Sufl. We report transport efficiencies of 60-80% for nanomolar pre-SufI concentrations. Dissipation of the pH gradient does not reduce pre-SufI transport efficiency. Instead, pre-SufI transport requires at least two electrical potential (Delta psi)-dependent steps that differ in both the duration and minimum magnitude of the required Delta psi. The data are consistent with a model in which a substantial Delta psi of short duration is required for an early transport step, and in which a small Delta psi of long duration is necessary to drive a later transport step.
引用
收藏
页码:87 / 99
页数:13
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