AQUEOUS TWO-PHASE EXTRACTION FOR THE PURIFICATION OF ALKALINE AGARASES FROM CULTURE EXTRACTS OF Pseudomonas aeruginosa AG LSL-11

被引：4

作者：

Koti, Basawaraj A. ^{[1
]}

Lakshmikanth, M. ^{[2
]}

Manohar, S. ^{[3
]}

Lalitha, J. ^{[1
]}

机构：

[1] Gulbarga Univ, Dept Biochem, Gulbarga 585106, Karnataka, India

[2] Heinrich Pette Inst Expt Virol & Immunol, Dept Cell Biol & Virol, Hamburg, Germany

[3] Avestagen Creator, ITPB, Bangalore, Karnataka, India

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2012年 / 42卷 / 04期

关键词：

agarase; aqueous two-phase system; Pseudomonas aeruginosa AG LSL-11; zymogram; PARTITION; SYSTEMS;

D O I：

10.1080/10826068.2011.623210

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The agarases were purified for the first time an using aqueous two-phase system (ATPS) consisting of polyethylene glycol (PEG) and phosphate salt. The three extracellular, alkaline agarases produced by Pseudomonas aeruginosa AG LSL-11 were efficiently extracted into the top PEG-rich layer. The influencing factors on the partition of agarases-molecular weight of the PEG, system pH, system temperature, and NaCl concentration-were investigated. All the factors were found to have a significant effect on the partition of agarases except NaCl. The optimal ATPS parameters for the partitioning and purification of agarases were found to be 12% PEG 600 and 11.9% (w/w) phosphate salt at pH 8.0 and 4 degrees C. All three agarases were concentrated in the top PEG phase with 6.19-fold purity and 71.21% recovery. The ATPS was found to be more convenient and economical than the conventional ion-exchange chromatography (IEC) method for extraction of three agarases and could be significantly employed for the purification of agarases from fermentation broth.

引用

页码：364 / 377

页数：14

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