Inhibitory effect on HT-29 colon cancer cells of a water-soluble polysaccharide obtained from highland barley

被引：52

作者：

Cheng, Dai ^{[1
]}

Zhang, Xinyu ^{[1
]}

Meng, Meng ^{[1
]}

Han, Lirong ^{[1
]}

Li, Caijiao ^{[1
]}

Hou, Lihua ^{[1
]}

Qi, Wentao ^{[2
]}

Wang, Chunling ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Key Lab Food Safety & Sanitat, Minist Educ, Tianjin, Peoples R China

[2] Acad State Adm Grain, 11 Baiwanzhuang Ave, Beijing 100037, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 92卷

关键词：

Polysaccharide; Barley; Human colon cancer; Anticancer; GRIFOLA-FRONDOSA; DIETARY FIBER; ACTIVATION; ANTITUMOR; APOPTOSIS; FRACTION; PATHWAY; MYCELIA; ORIGIN; DEATH;

D O I：

10.1016/j.ijbiomac.2016.06.099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A water-soluble polysaccharide (BP-1) was obtained from highland barley (Hordeum vulgare L.) by hot water extraction and purification of sepharose column chromatography. BP-1 had an average molecular weight of about 6.7 x 10(4) Da and was composed of glucose (Glc), xylose (Xyl), arabinose (Ara) and rhamnose (Rha) with a relative molar ratio of 8.82:1.92:1.50:1.00. It was found that BP-1 inhibited proliferation of human colon cancer cells (HT-29) in a time- and dose-dependent manner with half maximal inhibitory concentration at 48 h of 48.18 mu g/mL. Western blotting results showed that BP-1 enhanced the phosphorylation of c-Jun N-terminal kinase (JNK), processes associated with the reactive oxygen species (ROS) formation and inhibited nuclear factor-kappa B (NF-kappa B) translocation from cytoplasm into nucleus. Meanwhile, the BP-1-induced apoptosis was related to the regulation of apoptosis-associated proteins, such as B-cell lymphoma-2 (Bcl-2), release of cytochrome C from mitochondria to cytoplasm and activation of caspase-8 and caspase-9. These results suggest that BP-1-induced HT-29 apoptosis through ROS-JNK and NF-kappa B-mediated caspase pathways. (C) 2016 Published by Elsevier B.V.

引用

页码：88 / 95

页数：8

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