Endogenous Alpha-Synuclein Protein Analysis from Human Brain Tissues Using Single-Molecule Pull-Down Assay

被引：17
|
作者：
Je, Goun [1 ]
Croop, Benjamin [2 ]
Basu, Sambuddha [1 ]
Tang, Jialei [2 ]
Han, Kyu Young [2 ]
Kim, Yoon-Seong [1 ]
机构：
[1] Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Orlando, FL 32827 USA
[2] Univ Cent Florida, Coll Opt & Photon, CREOL, Orlando, FL 32816 USA
来源：
ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 24期
基金：
美国国家卫生研究院;
关键词：
PARKINSONS-DISEASE; SUBSTANTIA-NIGRA; LEWY BODY; COMPLEXES; AGGREGATION; OLIGOMERS; NEURONS; CELLS; STOICHIOMETRY; TOXICITY;
D O I：
10.1021/acs.analchem.7b04335
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Alpha-synudein (alpha-SYN) is a central molecule in Parkinson's disease pathogenesis. Despite several studies, the molecular nature of endogenous alpha-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of biospecimens. Here, we introduce alpha-SYN single-molecule pull-down (alpha-SIN SiMPull) assay combined with in vivo protein crosslinking to count individual alpha-SYN protein and assess its native oligomerization states from biological samples including human postmortem brains. This powerful single-molecule assay can be highly useful in diagnostic applications using various specimens for neurodegenerative diseases including Alzheimer's disease and Parkinson's disease.
引用
收藏
页码：13044 / 13048
页数：5
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