Histological and functional renal alterations caused by Bothrops alternatus snake venom: Expression and activity of Na+/K+-ATPase

被引：24

作者：

Linardi, Alessandra ^{[1
,2
]}

Rocha e Silva, Thomaz A. A. ^{[1
,2
]}

Miyabara, Elen H. ^{[3
]}

Franco-Penteado, Carla F. ^{[4
]}

Cardoso, Kiara C. ^{[1
]}

Boer, Patricia A. ^{[5
]}

Moriscot, Anselmo S. ^{[6
]}

Gontijo, Jose A. R. ^{[5
]}

Joazeiro, Paulo P. ^{[7
]}

Collares-Buzato, Carla B. ^{[7
]}

Hyslop, Stephen ^{[1
]}

机构：

[1] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Dept Farmacol, BR-13083970 Campinas, SP, Brazil

[2] Fac Ciencias Med Santa Casa Sao Paulo, Dept Ciencias Fisiol, BR-01221020 Sao Paulo, Brazil

[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, BR-05508900 Sao Paulo, Brazil

[4] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Hemoctr, BR-13083970 Campinas, SP, Brazil

[5] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Nucleo Med & Cirurgia Expt, BR-13083970 Campinas, SP, Brazil

[6] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, BR-05508900 Sao Paulo, Brazil

[7] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Histol & Embriol, BR-13083970 Campinas, SP, Brazil

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2011年 / 1810卷 / 09期

基金：

巴西圣保罗研究基金会;

关键词：

Acute renal failure; Bothrops alternatus; Collagen deposition; F-actin; Na+/K+-ATPase; Renal function; EXTENSOR DIGITORUM LONGUS; ISCHEMIA-INDUCED LOSS; EPITHELIAL POLARITY; JARARACUSSU VENOM; METALLOPROTEINASES; MECHANISMS; FAILURE; ASPER; REARRANGEMENT; PATHOGENESIS;

D O I：

10.1016/j.bbagen.2011.06.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Acute renal failure is a serious complication of human envenoming by Bothrops snakes. The ion pump Na+/K+-ATPase has an important role in renal tubule function, where it modulates sodium reabsorption and homeostasis of the extracellular compartment. Here, we investigated the morphological and functional renal alterations and changes in Na+/K+-ATPase expression and activity in rats injected with Bothrops alternatus snake venom. Methods: Male Wistar rats were injected with venom (0.8 mg/kg, iv.) and renal function was assessed 6.24, 48 and 72 h and 7 days post-venom. The rats were then killed and renal Na+/K+-ATPase activity was assayed based on phosphate release from ATP; gene and protein expressions were assessed by real time PCR and immunofluorescence microscopy, respectively. Results: Venom caused lobulation of the capillary tufts, dilation of Bowman's capsular space. F-actin disruption in Bowman's capsule and renal tubule brush border, and deposition of collagen around glomeruli and proximal tubules that persisted seven days after envenoming. Enhanced sodium and potassium excretion, reduced proximal sodium reabsorption, and proteinuria were observed 6 h post-venom, followed by a transient decrease in the glomerular filtration rate. Gene and protein expressions of the Na+/K+-ATPase alpha(1) subunit were increased 6 h post-venom, whereas Na+/K+-ATPase activity increased 6 h and 24 h post-venom. Conclusions: Bothrops alternatus venom caused marked morphological and functional renal alterations with enhanced Na+/K+-ATPase expression and activity in the early phase of renal damage. General significance: Enhanced Na+/K+-ATPase activity in the early hours after envenoming may attenuate the renal dysfunction associated with venom-induced damage. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：895 / 906

页数：12

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