Phosphorus deficiency impairs early nodule functioning and enhances proton release in roots of Medicago truncatula L.

被引:165
作者
Tang, C
Hinsinger, P
Drevon, JJ
Jaillard, B
机构
[1] INRA, UMR Sol & Environm, F-34060 Montpellier 1, France
[2] Univ Western Australia, Ctr Legumes Mediterranean Agr Soil Sci & Plant Nu, Crawley 6009, Australia
关键词
Medicago truncatula L. (medic); P deficiency; C2H2-ID; nitrogenase activity; proton release; cation-anion balance; open-flow system;
D O I
10.1006/anbo.2001.1440
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of phosphorus supply (1-15 muM) on proton release and the role of P in symbiotic nitrogen fixation in medic (Medicago truncatula L. 'Jemalong') was investigated. As P concentration in the nutrient solution increased, shoot and root growth increased by 19 and 15 %, respectively by day 35, with maximal growth at 4 muM P. 4 P concentration of 15 muM appeared to be toxic to plants. Phosphorus supply had no influence on nodule formation bq day 12 but increased nodule number by day 35. Nitrogenase activity was estimated by in situ measurement of acetylene reduction activity (ARA) in an open-flow system. During the assay, a C2H2-induced decline of ARA was observed under all P concentrations except 4 muM. Specific ARA (per unit nodule weight) doubled when P supply was increased from 1 to 8 muM. This effect of P was much greater than the effects of P on nodulation and host plant growth. Concentrations of excess cations in plants decreased with increasing P concentration in the nutrient solution. Phosphorus deficiency-stimulated uptake of excess cations over anions by the plants and hence enhanced proton release. The results suggest that P plays a direct role in nodule functioning in medic and that P deficiency increases acidification which may facilitate P acquisition. (C) 2001 Annals of Botany Company.
引用
收藏
页码:131 / 138
页数:8
相关论文
共 31 条
[1]  
*AOAC, 1975, OFF METH AN
[2]  
BEHI O, 2000, P 1 INT S P SOIL PLA, P57
[3]   GROWTH AND NODULATION OF TROPICAL FOOD LEGUMES IN DILUTE-SOLUTION CULTURE [J].
BELL, RW ;
EDWARDS, DG ;
ASHER, CJ .
PLANT AND SOIL, 1990, 122 (02) :249-258
[4]   Dynamics of phosphorus in the rhizosphere of maize and rape grown on synthetic, phosphated calcite and goethite [J].
Bertrand, I ;
Hinsinger, P ;
Jaillard, B ;
Arvieu, JC .
PLANT AND SOIL, 1999, 211 (01) :111-119
[5]   EFFECTS OF PHOSPHORUS ON SEED YIELDS OF SUBTERRANEAN CLOVER, SERRADELLA AND ANNUAL MEDICS [J].
BOLLAND, MDA .
AUSTRALIAN JOURNAL OF EXPERIMENTAL AGRICULTURE, 1985, 25 (03) :595-602
[6]   Phosphorus deficiency increases the argon-induced decline of nodule nitrogenase activity in soybean and alfalfa [J].
Drevon, JJ ;
Hartwig, UA .
PLANTA, 1997, 201 (04) :463-469
[7]  
DREVON JJ, 1988, PLANT PHYSIOL BIOCH, V26, P73
[8]  
EWING MA, 1991, THESIS U W AUSTR AUS
[9]   PHOSPHORUS REQUIREMENTS AND NITROGEN ACCUMULATION BY 3 MUNGBEAN (VIGNA-RADIATA (L) WELZEK) CULTIVARS [J].
GUNAWARDENA, SFBN ;
DANSO, SKA ;
ZAPATA, F .
PLANT AND SOIL, 1992, 147 (02) :267-274
[10]   DISTRIBUTION OF PHOSPHORUS IN NODULATED WHITE CLOVER PLANTS [J].
HART, AL .
JOURNAL OF PLANT NUTRITION, 1989, 12 (02) :159-171