Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles

被引：66

作者：

Merckx, Greet ^{[1
]}

Hosseinkhani, Baharak ^{[1
]}

Kuypers, Soren ^{[1
]}

Deville, Sarah ^{[1
,2
]}

Irobi, Joy ^{[1
]}

Nelissen, Inge ^{[2
]}

Michiels, Luc ^{[1
]}

Lambrichts, Ivo ^{[1
]}

Bronckaers, Annelies ^{[1
]}

机构：

[1] UHasselt Hasselt Univ, Fac Med & Life Sci, Biomed Res Inst BIOMED, Agoralaan, B-3590 Diepenbeek, Belgium

[2] Flemish Inst Technol Res VITO, Hlth Dept, B-2400 Mol, Belgium

来源：

CELLS | 2020年 / 9卷 / 02期

关键词：

extracellular vesicles; bone marrow-derived mesenchymal stromal cells; dental pulp stromal cells; angiogenesis; STEM-CELLS; PROMOTE ANGIOGENESIS; ENHANCE ANGIOGENESIS; INDUCE ANGIOGENESIS; IN-VITRO; EXOSOMES; IMPROVE; GROWTH; REGENERATION; INHIBITION;

D O I：

10.3390/cells9020312

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Blood vessel formation or angiogenesis is a key process for successful tooth regeneration. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) possess paracrine proangiogenic properties, which are, at least partially, induced by their extracellular vesicles (EVs). However, the isolation of BM-MSCs is associated with several drawbacks, which could be overcome by MSC-like cells of the teeth, called dental pulp stromal cells (DPSCs). This study aims to compare the angiogenic content and functions of DPSC and BM-MSC EVs and conditioned medium (CM). The angiogenic protein profile of DPSC- and BM-MSC-derived EVs, CM and EV-depleted CM was screened by an antibody array and confirmed by ELISA. Functional angiogenic effects were tested in transwell migration and chicken chorioallantoic membrane assays. All secretion fractions contained several pro- and anti-angiogenic proteins and induced in vitro endothelial cell motility. This chemotactic potential was higher for (EV-depleted) CM, compared to EVs with a stronger effect for BM-MSCs. Finally, BM-MSC CM, but not DPSC CM, nor EVs, increased in ovo angiogenesis. In conclusion, we showed that DPSCs are less potent in relation to endothelial cell chemotaxis and in ovo neovascularization, compared to BM-MSCs, which emphasizes the importance of choice of cell type and secretion fraction for stem cell-based regenerative therapies in inducing angiogenesis.

引用

页数：21

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