Anti-glioma properties of DVL, a lectin purified from Dioclea violacea

被引：26

作者：

Nascimento, Ana Paula M. ^{[1
,2
]}

Knaut, Jhonatas L. ^{[3
]}

Rieger, Debora K. ^{[4
,5
]}

Wolin, Ingrid A. V. ^{[1
,2
]}

Heinrich, Isabella A. ^{[3
]}

Mann, Josiane ^{[3
]}

Juarez, Andrea V. ^{[6
,7
]}

Sosa, Liliana del V. ^{[6
,7
]}

Lucia De Paul, Ana ^{[6
,7
]}

Moreira, Cleane G. ^{[8
]}

Silva, Ivanice B. ^{[8
]}

Nobre, Clareane S. ^{[8
]}

Osterne, Vinicius J. S. ^{[8
]}

Nascimento, Kyria S. ^{[8
]}

Cavada, Benildo S. ^{[8
]}

Leal, Rodrigo B. ^{[1
,2
,3
]}

机构：

[1] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Bioquim, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Programa Posgrad Bioquim, BR-88040900 Florianopolis, SC, Brazil

[3] Univ Fed Santa Catarina, Ctr Ciencias Biol, Programa Posgrad Neurociencias, BR-88040900 Florianopolis, SC, Brazil

[4] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Nutr, BR-88040900 Florianopolis, SC, Brazil

[5] Univ Fed Santa Catarina, Ctr Ciencias Biol, Programa Posgrad Nutr, BR-88040900 Florianopolis, SC, Brazil

[6] Univ Nacl Cordoba, Fac Ciencias Med, Ctr Microscopia Elect, Ciudad Univ, RA-5000 Cordoba, Argentina

[7] Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Inst Invest Ciencias Salud INICSA, Cordoba, Argentina

[8] Univ Fed Ceara, Dept Bioquim & Biol Mol, BioMol Lab, BR-60440970 Fortaleza, Ceara, Brazil

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 120卷

关键词：

Lectin; Autophagy; Glioma; Dioclea violacea; DVL; ConA; A-INDUCED AUTOPHAGY; CONCANAVALIN-A; CRYSTAL-STRUCTURE; PLANT-LECTINS; APOPTOSIS; GLIOBLASTOMA; GLIOMA; ASSAY; INDUCTION; MECHANISM;

D O I：

10.1016/j.ijbiomac.2018.08.106

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect and antitumor property. The results from this work showed the effects of the lectin extracted from the Dioclea violacea plant (DVL) on the C6 rat glioma cell line. DVL treatment was able to induce caspase-3 activation, apoptotic cell death and cellular membrane damage. Furthermore, DVL decreased mitochondrial membrane potential and increased the number of acidic vesicles and cleavage of LC3, indicating activation of autophagic processes. DVL also significantly inhibited cell migration. Compared to ConA, a well studied lectin extracted from Canavalia ensiformes seeds, some effects of DVL were more potent, including decreasing C6 glioma cell viability and migration ability. Taken together, the results suggest that DVL can induce glioma cell death, autophagy and inhibition of cell migration, displaying potential anti-glioma activity. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：566 / 577

页数：12

共 23 条

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Nascimento, Ana Paula M.
Wolin, Ingrid A. V.
Welter, Priscilla G.
Heinrich, Isabella A.
Zanotto-Filho, Alfeu
Osterne, Vinicius J. S.
Lossio, Claudia F.
Silva, Mayara T. L.
Nascimento, Kyria S.
Cavada, Benildo S.
Leal, Rodrigo B.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 134 : 660 - 672
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[7] Anti-glioma Efficacy and Mechanism of Action of Tripolinolate A from Tripolium pannonicum
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[8] Multiparametric Assessment of the Anti-glioma Properties of OKN007 by Magnetic Resonance Imaging
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