A method for determining the mutual diffusion coefficient of molecular solutes based on surface plasmon resonance sensing

被引：10

作者：

Loureiro, F. C. C. L. ^{[1
]}

Neto, A. G. S. Barreto ^{[1
]}

Moreira, C. S. ^{[1
]}

Lima, A. M. N. ^{[1
]}

Neff, H. ^{[1
,2
]}

机构：

[1] Univ Fed Campina Grande, Dept Elect Engn, Ctr Elect Engn & Informat, BR-58429900 Campina Grande, PB, Brazil

[2] NE Ctr Strateg Res CETENE, Syst & Integrated Circuits Lab LINCS, Recife, PE, Brazil

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 154卷 / 02期

关键词：

Microfluidic; Diffusivity; Surface plasmon resonance sensing; Finite element simulation; Molecular transport; SCALING LAWS; ETHANOL; SENSORS; FLOW; NMR;

D O I：

10.1016/j.snb.2010.02.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An experimental method, combining surface plasmon resonance sensing and microfluidics, to determine the mutual diffusion coefficient of molecular solutes, as ethanol and bovine serum albumin, is presented. Representative refractive index variations of analyte samples over time, and associated dynamic solute concentration profiles, respectively, have been employed to access molecular transport parameters. From both, Fick's diffusion length and Taylor's pulse dispersion methods, solute and solvent mutual diffusion coefficients for diluted ethanol and concentrated protein aqueous solutions have been obtained. Additionally, the dynamic behavior and geometry effects of molecular transport have been exploited using finite element simulations for the 3-dimensional case and confirmed experimentally. The numerical simulation also addresses the influence of temperature effects. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：129 / 136

页数：8

共 21 条

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[Anonymous], NIST CHEM WEBBOOK

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