Functional recovery after spinal cord injury in mice through activation of microglia and dendritic cells after IL-12 administration

被引:49
作者
Yaguchi, Masae [1 ]
Ohta, Shigeki [1 ]
Toyama, Yoshiaki [2 ]
Kawakami, Yutaka [3 ]
Toda, Masahiro [1 ,4 ]
机构
[1] Keio Univ, Sch Med, Neuroimmunol Res Grp, Shinjuku Ku, Keio, Japan
[2] Keio Univ, Sch Med, Dept Orthopaed Surg, Keio, Japan
[3] Keio Univ, Sch Med, Inst Adv Med Res, Div Cellular Signaling, Tokyo, Japan
[4] Keio Univ, Sch Med, Dept Neurosurg, Tokyo 160, Japan
关键词
IL-12; microglia; spinal cord injury; dendritic cells; BDNF; neurogenesis;
D O I
10.1002/jnr.21658
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have previously reported that the transplantation of dendritic cells (DCs) brings about functional recovery after spinal cord injury in mice through the activation of endogenous microglia/macrophages and neural stem/progenitor cells. In this study, the effect of interleukin-12 (IL-12), which is secreted from DCs, was evaluated for the treatment of spinal cord injury in mice. Administration of IL-12 into the injured site significantly increased the number of activated microglia/macrophages and DCs as well as the expression of brain-derived neurotrophic factor surrounding the lesion site. Immunohistochemical analyses showed that de novo neurogenesis and remyelination were induced by IL-12 treatment. Furthermore, an open field test using Basso-Beattie-Brenham scoring revealed a significant improvement of locomotor function in mice treated with IL-12. These results suggest that IL-12 administration into the injured spinal cord results in a functional recovery through the activation of microglia/macrophages and DCs. (C) 2008 Wiley-Liss, Inc.
引用
收藏
页码:1972 / 1980
页数:9
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