Differential toxicity of Mn2+ and Mn3+ to rat liver tissues: Oxidative damage, membrane fluidity and histopathological changes

被引：30

作者：

Huang, Peili ^{[1
]}

Li, Guojun ^{[1
,2
]}

Chen, Chunxia ^{[1
]}

Wang, Hui ^{[1
]}

Han, Ying ^{[1
]}

Zhang, Shuhua ^{[1
]}

Xiao, Yang ^{[1
]}

Zhang, Man ^{[1
]}

Liu, Na ^{[1
]}

Chu, Jinhua ^{[1
]}

Zhang, Long ^{[3
]}

Sun, Zhiwei ^{[1
]}

机构：

[1] Capital Med Univ, Sch Publ Hlth & Family Med, Dept Toxicol & Sanit Chem, Beijing 100069, Peoples R China

[2] Beijing Res Ctr Prevent Med, Beijing 100013, Peoples R China

[3] Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China

来源：

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY | 2012年 / 64卷 / 03期

关键词：

Manganese; Oxidative damage; Membrane fluidity; Histopathology; Liver; SUPEROXIDE-DISMUTASE; MANGANESE; STRESS; COPPER; IRON; ZINC;

D O I：

10.1016/j.etp.2010.08.006

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Toxicity due to overexposure to manganese (Mn) is becoming increasingly prevalent. Mn-induced neurodegenerative toxicity has been demonstrated, but little is known concerning the adverse effects of the element on the liver. Under physiological conditions, manganese primarily exists as divalent manganese (Mn2+) and trivalent manganese (Mn3+). The present study was designed to evaluate and compare the effects of Mn2+ and Mn3+ on oxidative hepatic damage, membrane fluidity and histopathological changes in rats. Rats exposed to Mn2+ or Mn3+ (2.0 mg Mn/kg body weight) showed significant inhibition of superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity, as well as decreased levels of glutathione (GSH) and increased levels of malondialdehyde (MDA) in liver tissues. We also showed a significant inhibition of SOD activity and increased MDA levels in hepatocyte nuclei. We also observed reduced Na+,K+-ATPase activity, increased MDA levels and decreased plasma membrane fluidity, which was accompanied by an increase of fluorescence anisotropy (r) values, in hepatic plasma membranes. In addition, Mn2+ and Mn3+ both caused histopathological changes, such as mononuclear cell infiltration, congestion, enlargement of the veins and sinusoids, hepatocellular damage, necrotic changes, mitochondrial hyperplasia, swelling and vacuolization, as determined by light and electron microscopy. Taken together, these data suggest that both Mn2+ and Mn3+ inhibit the normal physiological functioning of the liver. Under the experimental conditions used, the adverse effects of Mn2+ were more severe than those of Mn3+. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：197 / 203

页数：7

共 34 条

[1] MANGANESE-INDUCED REACTIVE OXYGEN SPECIES - COMPARISON BETWEEN MN+2 AND MN+3 [J].

ALI, SF ;

DUHART, HM ;

NEWPORT, GD ;

LIPE, GW ;

SLIKKER, W .

NEURODEGENERATION, 1995, 4 (03) :329-334

[2] MANGANESE POISONING AND THE ATTACK OF TRIVALENT MANGANESE UPON CATECHOLAMINES [J].

ARCHIBALD, FS ;

TYREE, C .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 256 (02) :638-650

[3] Manganese: Recent advances in understanding its transport and neurotoxicity [J].

Aschner, Michael ;

Guilarte, Tomas R. ;

Schneider, Jay S. ;

Zheng, Wei .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2007, 221 (02) :131-147

[4] Role of glutathione, lipid peroxidation and antioxidants on acute bile-duct obstruction in the rat [J].

Barón, V ;

Muriel, P .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1999, 1472 (1-2) :173-180

[5] NUCLEI FROM RAT LIVER - ISOLATION METHOD THAT COMBINES PURITY WITH HIGH YIELD [J].

BLOBEL, G ;

POTTER, VR .

SCIENCE, 1966, 154 (3757) :1662-&

[6] Manganese exposure: Neuropsychological and neurological symptoms and effects in welders [J].

Bowler, Rosemarie M. ;

Gysens, Sabine ;

Diamond, Emily ;

Nakagawa, Sanae ;

Drezgic, Marija ;

Roels, Harry A. .

NEUROTOXICOLOGY, 2006, 27 (03) :315-326

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] The Nrf2 transcription factor contributes to the induction of alpha-class GST isoenzymes in liver of acute cadmium or manganese intoxicated rats:: Comparison with the toxic effect on NAD(P)H:quinone reductase [J].

Casalino, Elisabetta ;

Calzaretti, Giovanna ;

Landriscina, Matteo ;

Sblano, Cesare ;

Fabiano, Annarita ;

Landriscina, Clemente .

TOXICOLOGY, 2007, 237 (1-3) :24-34

[9] Differential cytotoxicity of Mn(II) and Mn(III): Special reference mitochondrial [Fe-S] containing enzymes [J].

Chen, JY ;

Tsao, GC ;

Zhao, QQ ;

Zheng, W .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2001, 175 (02) :160-168

[10]

CHONG PLG, 1985, J BIOL CHEM, V260, P4484

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