Polyelectrolyte-mediated increase of biofilm mass formation

被引：17

作者：

Bucki, Robert ^{[1
,2
]}

Niemirowicz, Katarzyna ^{[1
]}

Wnorowska, Urszula ^{[1
]}

Watek, Marzena ^{[3
]}

Byfield, Fitzroy J. ^{[4
,5
]}

Cruz, Katrina ^{[4
,5
]}

Wroblewska, Marta ^{[6
]}

Janmey, Paul A. ^{[4
,5
]}

机构：

[1] Med Univ Bialystok, Dept Microbiol & Nanobiomed Engn, PL-15222 Bialystok, Poland

[2] Jan Kochanowski Univ, Fac Hlth Sci, Dept Physiol Pathophysiol & Microbiol Infect, Kielce, Poland

[3] Holy Cross Oncol Ctr Kielce, Dept Hematol, Kielce, Poland

[4] Univ Penn, Dept Phys, Philadelphia, PA 19104 USA

[5] Univ Penn, Inst Med & Engn, Philadelphia, PA 19104 USA

[6] Med Univ Warsaw, Dept Dent Microbiol, Warsaw, Poland

来源：

BMC MICROBIOLOGY | 2015年 / 15卷

关键词：

Bacteria; C; albicans; Filamentous bacteriophages; DNA; F-actin; Biofilm mass; PSEUDOMONAS-AERUGINOSA; EXTRACELLULAR DNA; SWARMING MOTILITY; F-ACTIN; CERAGENIN CSA-13; RESISTANCE; RELEASE; PEPTIDE; BUNDLES; LL-37;

D O I：

10.1186/s12866-015-0457-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Biofilm formation is associated with various aspects of bacterial and fungal infection. This study was designed to assess the impact of diverse natural polyelectrolytes, such as DNA, F-actin, neurofilaments (NFs), vimentin and purified Pf1 bacteriophage on biofilm formation and swarming motility of select pathogens including Pseudomonas aeruginosa associated with lung infections in CF patients. Results: The bacteriophage Pf1 (1 mg/ml) significantly increased biofilm mass produced by Pseudomonas aeruginosa P14, Escherichia coli RS218 and Bacillus subtilis ATCC6051. DNA, F-actin, NFs and Pf1 also increased biofilm mass of the fungal C. albicans 1409 strain. Addition of F-actin, DNA or Pf1 bacteriophage to 0.5% agar plates increased swarming motility of Pseudomonas aeruginosa Xen5. Conclusions: The presence of polyelectrolytes at infection sites is likely to promote biofilm growth and bacterial swarming.

引用

页数：10

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