Use of Real-time PCR to Detect Canine Parvovirus in Feces of Free-ranging Wolves

被引：11

作者：

Mech, L. David ^{[1
]}

Almberg, Emily S. ^{[2
]}

Smith, Douglas ^{[3
]}

Goyal, Sagar ^{[4
]}

Singer, Randall S. ^{[4
]}

机构：

[1] US Geol Survey, No Prairie Wildlife Res Ctr, Jamestown, ND 58401 USA

[2] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, St Paul, MN 55108 USA

[3] Natl Pk Serv, Yellowstone Ctr Resources, Yellowstone Natl Pk, WY 82190 USA

[4] Univ Minnesota, Coll Vet Med, St Paul, MN 55108 USA

来源：

JOURNAL OF WILDLIFE DISEASES | 2012年 / 48卷 / 02期

关键词：

Canine parvovirus; Canis lupus; feces; real-time PCR; wolf;

D O I：

10.7589/0090-3558-48.2.473

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10(4) 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

引用

页码：473 / 476

页数：4

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