Haploidentical hematopoietic stem cell transplantation in children with high-risk hematologic malignancies: outcomes with two different strategies for GvHD prevention. Ex vivo T-cell depletion and post-transplant cyclophosphamide: 10 years of experience at a single center

被引:26
作者
Dufort, G. [1 ]
Castillo, L. [1 ]
Pisano, S. [2 ]
Castiglioni, M. [1 ]
Carolina, P. [1 ]
Andrea, I. [1 ]
Simon, E. [1 ]
Zuccolo, S. [1 ]
Schelotto, M. [1 ]
Morosini, F. [1 ]
Pereira, I. [1 ]
Amarillo, P. [1 ]
Silveira, A. [3 ]
Guerrero, L. [4 ]
Ferreira, V. [4 ]
Tiscornia, A. [2 ]
Mezzano, R. [2 ]
Lemos, F. [2 ]
Boggia, B. [2 ]
Quarnetti, A. [4 ]
Decaro, J. [5 ]
Dabezies, A. [1 ]
机构
[1] Ctr Hosp Pereira Rosell, Pediat Hematooncol Dept, Bulevar Artigas 1556, Montevideo 11600, Uruguay
[2] Ctr Hosp Pereira Rosell, Dept Transfus Med, Montevideo, Uruguay
[3] Ctr Hosp Pereira Rosell, Dept Stat, Montevideo, Uruguay
[4] Ctr Hosp Pereira Rosell, Radiotherapy Dept, Montevideo, Uruguay
[5] Asociac Espanola, Transfus Med Dept, Montevideo, Uruguay
关键词
VERSUS-HOST-DISEASE; IDENTICAL SIBLING TRANSPLANTATION; MARROW-TRANSPLANTATION; ACUTE-LEUKEMIA; BLOOD; DONORS; FEASIBILITY; MORTALITY; URUGUAY; GRAFTS;
D O I
10.1038/bmt.2016.161
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Forty patients with high-risk hematologic malignancies, median age 9 years, underwent haploidentical-HSCT from April 2005 to April 2015. Seventeen patients were transplanted with CD3-depleted PBSCs by negative selection (TCD group) following a reduced-intensity conditioning regimen (RIC), and 23 patients received T-cell-replete PBSCs followed by post-transplantation cyclophosphamide (PT-Cy group) after myeloablative conditioning (n = 16) or RIC (n = 7). Outcomes are reported for the TCD and PT-Cy recipients, respectively. Engraftment was achieved in 88% versus 100%. Median time to neutrophils > 500/mu L was 10 days versus 15 days. Platelets >20 000/mu L occurred at a median of 16 days versus 20 days, respectively. Transplant-related mortality (TRM) was 24% versus 26% at 1 year. The cumulative incidence (CI) of grade III - IV acute GvHD was 7% versus 5%, and chronic GvHD 9% versus 53% (P = 0.029). Relapse at 2 years was 31% versus 24%. Actuarial overall survival rates at 2 years were 47% versus 48%. Causes of death were infections (n = 3), sinusoidal obstructive syndrome (n = 4), acute GvHD (n = 2) and relapse (n = 9). These results indicate that haploidentical-HSCT is feasible in Uruguay. The TRM rate is of concern and should be the focus of continuing attention. Chronic GvHD risk was higher in the PT-Cy approach, so modifications are justified.
引用
收藏
页码:1354 / 1360
页数:7
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