Anticancer activity of the supercritical extract of Brazilian green propolis and its active component, artepillin C: Bioinformatics and experimental analyses of its mechanisms of action

被引：45

作者：

Bhargava, Priyanshu ^{[1
,2
]}

Grover, Abhinav ^{[3
]}

Nigam, Nupur ^{[1
]}

Kaul, Ashish ^{[1
,4
]}

Doi, Motomichi ^{[1
,2
]}

Ishida, Yoshiyuki ^{[5
]}

Kakuta, Hanzo ^{[6
]}

Kaul, Sunil C. ^{[1
]}

Terao, Keiji ^{[5
,7
]}

Wadhwa, Renu ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, DAILAB, Cent 5-41,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan

[2] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058575, Japan

[3] Jawaharlal Nehru Univ, Sch Biotechnol, New Delhi 110067, India

[4] Univ Tsukuba, Sch Integrat & Global Majors, Tsukuba, Ibaraki 3058577, Japan

[5] CycloChem Co Ltd, Kobe, Hyogo 6500047, Japan

[6] Fujimi Bee House Co Ltd, Shiki 3530003, Japan

[7] Kobe Univ, Grad Sch Med, Kobe, Hyogo 6500017, Japan

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2018年 / 52卷 / 03期

关键词：

Brazilian green propolis; supercritical extract; cyclodextrin conjugate; p53-mortalin complex; abrogation; anticancer; CHEMICAL DIVERSITY; ANTIBACTERIAL; ANTIOXIDANT; GROWTH; CELLS; IDENTIFICATION; APOPTOSIS; MORTALIN; GROMACS; AGENTS;

D O I：

10.3892/ijo.2018.4249

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Propolis, a resinous substance collected by honeybees by mixing their saliva with plant sources, including tree bark and leaves and then mixed with secreted beeswax, possesses a variety of bioactivities. Whereas caffeic acid phenethyl ester (CAPE) has been recognized as a major bioactive ingredient in New Zealand propolis, Brazilian green propolis, on the other hand, possesses artepillin C (ARC). In this study, we report that, similar to CAPE, ARC docks into and abrogates mortalin-p53 complexes, causing the activation of p53 and the growth arrest of cancer cells. Cell viability assays using ARC and green propolis-supercritical extract (GPSE) revealed higher cytotoxicity in the latter, supported by nuclear translocation and the activation of p53. Furthermore, in vivo tumor suppression assays using nude mice, we found that GPSE and its conjugate with cyclodextrin (CD) possessed more potent anticancer activity than purified ARC. GPSE-CD may thus be recommended as a natural, effective and economic anticancer amalgam.

引用

页码：925 / 932

页数：8

共 48 条

[1] Applications of Supercritical Fluid Extraction (SFE) of Palm Oil and Oil from Natural Sources [J].