An automated imaging system for radiation biodosimetry

被引：24

作者：

Garty, Guy ^{[1
]}

Bigelow, Alan W. ^{[1
]}

Repin, Mikhail ^{[2
]}

Turner, Helen C. ^{[2
]}

Bian, Dakai ^{[3
]}

Balajee, Adayabalam S. ^{[2
]}

Lyulko, Oleksandra V. ^{[1
]}

Taveras, Maria ^{[2
]}

Yao, Y. Lawrence ^{[3
]}

Brenner, David J. ^{[2
]}

机构：

[1] Columbia Univ, Radiol Res Accelerator Facil, Dept Radiat Oncol, Irvington, NY 10533 USA

[2] Columbia Univ, Dept Radiat Oncol, Ctr Radiol Res, New York, NY 10032 USA

[3] Columbia Univ, Dept Mech Engn, New York, NY 10027 USA

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2015年 / 78卷 / 07期

基金：

美国国家卫生研究院;

关键词：

dicentrics; mBAND; -H2AX; micronuclei; fluorescence microscopy; sCMOS;

D O I：

10.1002/jemt.22512

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

We describe here an automated imaging system developed at the Center for High Throughput Minimally Invasive Radiation Biodosimetry. The imaging system is built around a fast, sensitive sCMOS camera and rapid switchable LED light source. It features complete automation of all the steps of the imaging process and contains built-in feedback loops to ensure proper operation. The imaging system is intended as a back end to the RABiTa robotic platform for radiation biodosimetry. It is intended to automate image acquisition and analysis for four biodosimetry assays for which we have developed automated protocols: The Cytokinesis Blocked Micronucleus assay, the -H2AX assay, the Dicentric assay (using PNA or FISH probes) and the RABiT-BAND assay. Microsc. Res. Tech. 78:587-598, 2015. (c) 2015 Wiley Periodicals, Inc.

引用

页码：587 / 598

页数：12

共 6 条

[1]

[Anonymous], RAD RES, V175, P282

[2] The RABiT: high-throughput technology for assessing global DSB repair [J].

Turner, Helen C. ;

Sharma, P. ;

Perrier, J. R. ;

Bertucci, A. ;

Smilenov, L. ;

Johnson, G. ;

Taveras, M. ;

Brenner, D. J. ;

Garty, G. .

RADIATION AND ENVIRONMENTAL BIOPHYSICS, 2014, 53 (02) :265-272

[3] Automated gamma-H2AX focus scoring method for human lymphocytes after ionizing radiation exposure [J].

Valente, M. ;

Voisin, P. ;

Laloi, P. ;

Roy, L. ;

Roch-Lefevre, S. .

RADIATION MEASUREMENTS, 2011, 46 (09) :871-876

[4] An automated scoring procedure for the micronucleus test by image analysis [J].

Varga, D ;

Johannes, T ;

Jainta, S ;

Schuster, S ;

Schwarz-Boeger, U ;

Kiechle, M ;

Garcia, BP ;

Vogel, W .

MUTAGENESIS, 2004, 19 (05) :391-397

[5] Biological dosimetry by the triage dicentric chromosome assay - Further validation of international networking [J].

Wilkins, Ruth C. ;

Romm, Horst ;

Oestreicher, Ursula ;

Marro, Leonora ;

Yoshida, Mitsuaki A. ;

Suto, Y. ;

Prasanna, Pataje G. S. .

RADIATION MEASUREMENTS, 2011, 46 (09) :923-928

[6] Multispot Live-Image Autofocusing for High-Throughput Microscopy of Fluorescently Stained Bacteria [J].

Zeder, M. ;

Pernthaler, J. .

CYTOMETRY PART A, 2009, 75A (09) :781-788

← 1 →