Comparative analysis of markers of cell proliferation in canine mast cell tumours according to current classifications

被引：11

作者：

Kandefer-Gola, M. ^{[1
]}

Madej, J. A. ^{[1
]}

Dzimira, S. ^{[1
]}

Nowak, M. ^{[1
]}

Janus, I. ^{[1
]}

Ciaputa, R. ^{[1
]}

机构：

[1] Wroclaw Univ Environm & Life Sci, Div Pathomorphol & Vet Forens, Dept Pathol, PL-50375 Wroclaw, Poland

来源：

POLISH JOURNAL OF VETERINARY SCIENCES | 2015年 / 18卷 / 02期

关键词：

dog; Ki-67; Kiupel grading system; mast cell tumour; MCM-3; Patnaik grading system; PCNA; NUCLEAR ANTIGEN; MCM PROTEINS; SURVIVAL; PROGNOSIS; KI-67; DOGS;

D O I：

10.1515/pjvs-2015-0031

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The study aimed at immunohistochemical analysis of various markers of cell proliferation and comparison of the results with canine mast cell tumours grading systems according to the Patnaik and Kiupel. Tissue sections were stained using classical technique with haematoxylin and eosin, and immunohistochemical studies were performed with Ki-67, PCNA and MCM-3 antibodies. Additionally the mitotic index was assessed. Statistical analysis including rank correlation Spearman's and ANOVA Friedman analysis was performed. The significance was set at p<0.05. Expression of all examined antigens was detected. The results obtained allow concluding that there is a strong relationship between all the cell markers. However, due to the very strong response and positive reaction in the majority of tumours PCNA is not recommended as a prognostic indicator. Ki-67 and MCM-3 can be successfully used in the evaluation of canine mast cell tumours.

引用

页码：241 / 247

页数：7

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