Interconversion Between Intestinal Stem Cell Populations in Distinct Niches

被引：589

作者：

Takeda, Norifumi ^{[1
,2
,3
]}

Jain, Rajan ^{[1
,2
,3
]}

LeBoeuf, Matthew R. ^{[1
,4
]}

Wang, Qiaohong ^{[1
,2
,3
]}

Lu, Min Min ^{[2
]}

Epstein, Jonathan A. ^{[1
,2
,3
]}

机构：

[1] Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA

[2] Univ Penn, Perelman Sch Med, Penn Cardiovasc Inst, Philadelphia, PA 19104 USA

[3] Univ Penn, Perelman Sch Med, Inst Regenerat Med, Philadelphia, PA 19104 USA

[4] Univ Penn, Perelman Sch Med, Dept Dermatol, Philadelphia, PA 19104 USA

来源：

SCIENCE | 2011年 / 334卷 / 6061期

关键词：

PROGENITOR CELLS; MOUSE; MARKER; HOP; IDENTIFICATION; EXPRESSION; RENEWAL; PROTEIN; CANCER; GENE;

D O I：

10.1126/science.1213214

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intestinal epithelial stem cell identity and location have been the subject of substantial research. Cells in the +4 niche are slow-cycling and label-retaining, whereas a different stem cell niche located at the crypt base is occupied by crypt base columnar (CBC) cells. CBCs are distinct from +4 cells, and the relationship between them is unknown, though both give rise to all intestinal epithelial lineages. We demonstrate that Hopx, an atypical homeobox protein, is a specific marker of +4 cells. Hopx-expressing cells give rise to CBCs and all mature intestinal epithelial lineages. Conversely, CBCs can give rise to +4 Hopx-positive cells. These findings demonstrate a bidirectional lineage relationship between active and quiescent stem cells in their niches.

引用

页码：1420 / 1424

页数：5

共 23 条

[1] Identification of stem cells in small intestine and colon by marker gene Lgr5 [J].

Barker, Nick ;

van Es, Johan H. ;

Kuipers, Jeroen ;

Kujala, Pekka ;

van den Born, Maaike ;

Cozijnsen, Miranda ;

Haegebarth, Andrea ;

Korving, Jeroen ;

Begthel, Harry ;

Peters, Peter J. ;

Clevers, Hans .

NATURE, 2007, 449 (7165) :1003-U1

[2] Clonal analysis of mouse intestinal epithelial progenitors [J].

Bjerknes, M ;

Cheng, H .

GASTROENTEROLOGY, 1999, 116 (01) :7-14

[3] Generation of mTert-GFP mice as a model to identify and study tissue progenitor cells [J].

Breault, David T. ;

Min, Irene M. ;

Carlone, Diana L. ;

Farilla, Loredana G. ;

Ambruzs, Dana M. ;

Henderson, Daniel E. ;

Algra, Selma ;

Montgomery, Robert K. ;

Wagers, Amy J. ;

Hole, Nicholas .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (30) :10420-10425

[4] Hop is an unusual homeobox gene that modulates cardiac development [J].

Chen, F ;

Kook, H ;

Milewski, R ;

Gitler, AD ;

Lu, MM ;

Li, J ;

Nazarian, R ;

Schnepp, R ;

Jen, K ;

Biben, C ;

Runke, G ;

Mackay, JP ;

Novotny, J ;

Schwartz, RJ ;

Harvey, RP ;

Mullins, MC ;

Epstein, JA .

CELL, 2002, 110 (06) :713-723

[5] ORIGIN, DIFFERENTIATION AND RENEWAL OF 4 MAIN EPITHELIAL-CELL TYPES IN MOUSE SMALL INTESTINE .5. UNITARIAN THEORY OF ORIGIN OF 4 EPITHELIAL-CELL TYPES [J].

CHENG, H ;

LEBLOND, CP .

AMERICAN JOURNAL OF ANATOMY, 1974, 141 (04) :537-&

[6] Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP [J].

De Toni, Arianna ;

Zbinden, Marie ;

Epstein, Jonathan A. ;

Ruiz i Altaba, Ariel ;

Prochiantz, Alain ;

Caille, Isabelle .

NEURAL DEVELOPMENT, 2008, 3 (1)

[7] BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-β-catenin signaling [J].

He, XC ;

Zhang, JW ;

Tong, WG ;

Tawfik, O ;

Ross, J ;

Scoville, DH ;

Tian, Q ;

Zeng, X ;

He, X ;

Wiedemann, LM ;

Mishina, Y ;

Li, LH .

NATURE GENETICS, 2004, 36 (10) :1117-1121

[8] Coexistence of Quiescent and Active Adult Stem Cells in Mammals [J].

Li, Linheng ;

Clevers, Hans .

SCIENCE, 2010, 327 (5965) :542-545

[9] Mouse telomerase reverse transcriptase (mTert) expression marks slowly cycling intestinal stem cells [J].

Montgomery, Robert K. ;

Carlone, Diana L. ;

Richmond, Camilla A. ;

Farilla, Loredana ;

Kranendonk, Mariette E. G. ;

Henderson, Daniel E. ;

Baffour-Awuah, Nana Yaa ;

Ambruzs, Dana M. ;

Fogli, Laura K. ;

Algra, Selma ;

Breault, David T. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (01) :179-184

[10] CONTINUOUS LABELING STUDIES ON MOUSE SKIN AND INTESTINE [J].

POTTEN, CS ;

KOVACS, L ;

HAMILTON, E .

CELL AND TISSUE KINETICS, 1974, 7 (03) :271-283

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