Synchrotron FTIR Microspectroscopy of Single Natural Silk Fibers

被引：278

作者：

Ling, Shengjie ^{[1
]}

Qi, Zeming ^{[2
]}

Knight, David P. ^{[3
]}

Shao, Zhengzhong ^{[1
]}

Chen, Xin ^{[1
]}

机构：

[1] Fudan Univ, Adv Mat Lab, Dept Macromol Sci, Key Lab Mol Engn Polymers MOE, Shanghai 200433, Peoples R China

[2] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Peoples R China

[3] Oxford Biomat Ltd, Magdalen Ctr, Oxford OX4 4GA, England

来源：

BIOMACROMOLECULES | 2011年 / 12卷 / 09期

基金：

中国国家自然科学基金;

关键词：

BOMBYX-MORI SILK; X-RAY-DIFFRACTION; CONFORMATION TRANSITION KINETICS; SECONDARY STRUCTURE ESTIMATION; SPIDER SILK; SOLID-STATE; HETEROGENEOUS STRUCTURE; SPECTROSCOPIC ANALYSIS; MECHANICAL-PROPERTIES; FIBROIN;

D O I：

10.1021/bm2006032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synchrotron FTIR (S-FTIR) microspectroscopy was used to monitor the silk protein conformation in a range of single natural silk fibers (domestic and wild silkworm and spider dragline silk). With the selection of suitable aperture size, we obtained high-resolution S-FTIR spectra capable of semiquantitative analysis of protein secondary structures. For the first time, we have determined from S-FTTR the beta-sheet content in a range of natural single silk fibers, 28 +/- 4, 23 +/- 2, and 17 +/- 4% in Bombyx mori, Antheraea pernyi, and Nephila edulis silks, respectively. The trend of beta-sheet content in different silk fibers from the current study accords quite well with published data determined by MID, Raman, and C-13 NMR. Our results indicate that the, S-FTIR microspectroscopy method has considerable potential for the study of single natural silk fibers.

引用

页码：3344 / 3349

页数：6

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