Truly Nonionic Polymer Shells for the Encapsulation of Living Cells

被引：48

作者：

Carter, Jessica L. ^{[1
]}

Drachuk, Irina ^{[1
]}

Harbaugh, Svetlana ^{[2
]}

Kelley-Loughnane, Nancy ^{[2
]}

Stone, Morley ^{[2
]}

Tsukruk, Vladimir V. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA

[2] USAF, Res Lab, Directorate Human Effectiveness, Dayton, OH 45433 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 09期

关键词：

atomic force microscopy (AFM); biological applications of polymers; cell encapsulation; layer-by-layer assembly; polymer membranes; SOL-GEL ENCAPSULATION; ALGINATE HYDROGELS; ULTRATHIN FILMS; TANNIC-ACID; THIN-FILMS; LAYER; CAPSULES; MULTILAYERS; SURFACE; ARRAYS;

D O I：

10.1002/mabi.201100129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Engineering surfaces of living cells with natural or synthetic compounds can mediate intercellular communication and provide a protective barrier from hostile agents. We report on truly nonionic hydrogen-bonded LbL coatings for cell surface engineering. These ultrathin, highly permeable polymer membranes are constructed on living cells without the cationic component typically employed to increase the stability of LbL coatings. Without the cytotoxic cationic PEI pre-layer, the viability of encapsulated cells drastically increases to 94%, in contrast to 20% viability in electrostatically-bonded LbL shells. Moreover, the long-term growth of encapsulated cells is not affected, thus facilitating efficient function of protected cells in hostile environment.

引用

页码：1244 / 1253

页数：10

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