Production of reactive persulfide species in chronic obstructive pulmonary disease

被引：61

作者：

Numakura, Tadahisa ^{[1
]}

Sugiura, Hisatoshi ^{[1
]}

Akaike, Takaaki ^{[2
]}

Ida, Tomoaki ^{[2
]}

Fujii, Shigemoto ^{[2
]}

Koarai, Akira ^{[1
]}

Yamada, Mitsuhiro ^{[1
]}

Onodera, Katsuhiro ^{[1
]}

Hashimoto, Yuichiro ^{[1
]}

Tanaka, Rie ^{[1
]}

Sato, Kei ^{[1
]}

Shishikura, Yutaka ^{[1
]}

Hirano, Taizou ^{[1
]}

Yanagisawa, Satoru ^{[1
]}

Fujino, Naoya ^{[1
]}

Okazaki, Tatsuma ^{[1
]}

Tamada, Tsutomu ^{[1
]}

Hoshikawa, Yasushi ^{[3
]}

Okada, Yoshinori ^{[4
]}

Ichinose, Masakazu ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Dept Resp Med, Sendai, Miyagi, Japan

[2] Tohoku Univ, Grad Sch Med, Dep Environm Hlth Sci & Mol Toxicol, Sendai, Miyagi, Japan

[3] Fujita Hlth Univ, Sch Med, Dept Thorac Surg, Toyoake, Aichi, Japan

[4] Tohoku Univ, Inst Dev Aging & Canc, Dept Thorac Surg, Sendai, Miyagi, Japan

来源：

THORAX | 2017年 / 72卷 / 12期

基金：

日本学术振兴会;

关键词：

UNFOLDED PROTEIN RESPONSE; OXIDATIVE STRESS; CHEMICAL BIOLOGY; HYDROGEN-SULFIDE; COPD; HYDROPERSULFIDES; SULFHYDRATION; INFLAMMATION; H2S;

D O I：

10.1136/thoraxjnl-2016-209359

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Background Oxidative stress is a major aetiological factor driving chronic obstructive pulmonary disease (COPD). Recently recognised as potent antioxidants, reactive persulfide and polysulfide species are biosynthesised by cystathionine beta-synthase and cystathionine gamma-lyase. The production of reactive persulfide and polysulfide species in the lungs of patients with COPD remain unknown. Objectives The aim of this study was to examine the production of reactive persulfides and polysulfides, such as glutathione persulfide (GSSH), cysteine persulfide (CysSSH) and glutathione trisulfide (GSSSH), in lungresident cells and epithelial lining fluid (ELF) obtained from patients with mild to moderate COPD. Methods Lung tissues, primary lung cells, ELF and sputum were obtained. The amounts of reactive persulfides and polysulfides in the cells and ELF were measured by liquid chromatography-tandem mass spectrometry with beta-(4-hydroxyphenyl) ethyl iodoacetamide as a trapping agent for hydroper/ polysulfides. The amounts of synthases in the lung tissues, sputum and primary cells were quantified. Results The amounts of GSSH, CysSSH and GSSSH were decreased in the lung cells and ELF from patients with COPD. The amounts of reactive persulfides and polysulfides in the lung cells had a positive correlation with the degree of airflow limitation. By contrast, the amounts of the synthases were increased in the lung tissues and sputum cells of patients with COPD. Conclusions We have identified a decrease in reactive persulfide and polysulfide species in the lungs of patients with COPD. These data suggest that the newly detected antioxidants reactive persulfides and polysulfides could be associated with the redox balance in the lungs of patients with COPD.

引用

页码：1074 / 1083

页数：10

共 32 条

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