DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations

被引：29

作者：

Jiang, Yiting ^{[1
,2
]}

Miao, Peng ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China

[2] Univ Sci & Technol China, Hefei 230026, Peoples R China

来源：

RESEARCH | 2020年 / 2020卷

关键词：

MICRORNAS; PROLIFERATION; RECOGNITION; BIOMARKERS; GLIOMA;

D O I：

10.34133/2020/1091605

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Multiplex miRNA analysis is a fundamental issue for exploring a complex biological system and early diagnosis of miRNA-related diseases. Herein, we have developed a series of novel logic gates for miRNA analysis coupling DNA nanostructures and chameleon silver nanoclusters (AgNCs). DNA dumbbell structures are firstly designed with two independent nucleation sequences for AgNCs at the 5' and 3' ends, respectively. By introducing different miRNA inputs, separations of two AgNCs are controlled and the fluorescence property of AgNCs changes. By studying the ratiometric fluorescence responses, sensitive and selective analysis of multiple miRNAs can be achieved. The present work provides powerful tools for miRNA diagnostics and may also guide future DNA nanostructure-based logic gates.

引用

页数：8

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