Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol

被引:3
作者
Evazalipour, Mehdi [1 ]
Kozani, Pouya Safarzadeh [2 ,3 ]
Kozani, Pooria Safarzadeh [4 ]
Shabani, Sahar [5 ]
Soufi, Bahar Rezaei [6 ]
Zamani, Ehsan [5 ]
机构
[1] Guilan Univ Med Sci, Sch Pharm, Dept Pharmaceut Biotechnol, Rasht, Iran
[2] Guilan Univ Med Sci, Fac Paramed, Dept Med Biotechnol, Rasht, Iran
[3] Guilan Univ Med Sci, Med Biotechnol Res Ctr, Sch Nursing Midwifery & Paramed, Student Res Comm, Rasht, Iran
[4] Tarbiat Modares Univ, Fac Med Sci, Dept Med Biotechnol, Tehran, Iran
[5] Guilan Univ Med Sci, Sch Pharm, Dept Pharmacol & Toxicol, Rasht, Iran
[6] Guilan Univ Med Sci, Sch Pharm, Student Res Comm, Rasht, Iran
关键词
Acrylamide; Carvacrol; Oxidative Stress; Senescence; Fibroblasts; STRESS; ANTIOXIDANT; ACID;
D O I
10.5812/jjnpp.109399
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Stress-induced cellular senescence is a perpetual state of cell cycle arrest occurring in proliferating cells in response to stressful conditions. It is believed that oxidative stress plays a unique role in this process. As a reactive chemical compound that can induce oxidative stress, acrylamide is widely applied in several fields. Carvacrol is a liquid phenolic monoterpenoid found in essential oils of some plants and is known for its antioxidant and anticarcinogenic properties. Objectives: The current study aimed to evaluate the effects of carvacrol on oxidative stress and cellular senescence induced by acrylamide in the NIH 3T3 cell line. Methods: NIH 3T3 embryonic fibroblast cells were exposed to different concentrations of acrylamide, carvacrol, and H2O2 in a cell culture medium. The level of beta-galactosidase (SA-beta-gal) activity, as a marker of cellular senescence, was measured using staining and quantitative assays. Furthermore, to measure oxidative stress parameters, the content of glutathione and lipid peroxidation were determined. Results: Acrylamide could induce premature senescence evident by the elevated lipid peroxidation and SA-beta-gal activity and declined cell viability and glutathione. Moreover, carvacrol showed beneficial effects on both acrylamide- and H2O2-induced cellular senescence by significantly reversing or subsiding the effect of oxidative stress and mediating its consequences. Conclusions: It can be concluded that carvacrol has protective effects against oxidative cellular senescence induced by acrylamide in the NIH 3T3 cell line.
引用
收藏
页数:9
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