Ibuprofen metabolite profiling using a combination of SPE/column-trapping and HPLC-micro-coil NMR

被引：24
作者：
Djukovic, Danijel [1 ]
Appiah-Amponsah, Emmanuel [1 ]
Shanaiah, Narasimhamurthy [1 ]
Gowda, G. A. Nagana [1 ]
Henry, Ian [1 ]
Everly, Mike [1 ]
Tobias, Brian [2 ]
Raftery, Daniel [1 ]
机构：
[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[2] Pfizer Inc, San Diego, CA 92121 USA
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2008年 / 47卷 / 02期
关键词：
metabolite profiling; H-1; NMR; micro-coil; solid-phase extraction; ibuprofen;
D O I：
10.1016/j.jpba.2007.12.035
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC-nuclear magnetic resonance (HPLC-NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold. (c) 2008 Published by Elsevier B.V.
引用
收藏
页码：328 / 334
页数：7
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