Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry

被引：26

作者：

Palmer, Andrew D. ^{[2
]}

Griffiths, Rian ^{[1
]}

Styles, Iain ^{[3
]}

Claridge, Ela ^{[3
]}

Calcagni, Antonio ^{[4
]}

Bunch, Josephine ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England

[2] Univ Birmingham, PSIBS Doctoral Training Ctr, Birmingham, W Midlands, England

[3] Univ Birmingham, Sch Comp Sci, Birmingham B15 2TT, W Midlands, England

[4] Aston Univ, Sch Life & Hlth Sci, Birmingham B4 7ET, W Midlands, England

来源：

JOURNAL OF MASS SPECTROMETRY | 2012年 / 47卷 / 02期

基金：

英国工程与自然科学研究理事会;

关键词：

MALDI; Imaging; Sucrose; Cryo-protection; Eye; Histology; OCULAR LENS; PRODUCTS;

D O I：

10.1002/jms.2049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-mu m thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections. Copyright (C) 2012 John Wiley & Sons, Ltd.

引用

页码：237 / 241

页数：5

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