Evaluation of an indirect ELISA using recombinant granule antigen GRA1, GRA7 and soluble antigens for serodiagnosis of Toxoplasma gondii infection in chickens

被引：24
作者：
Sun, Xuefei [1 ]
Wang, Zedong [2 ]
Li, Jiping [1 ]
Wei, Feng [1 ]
Liu, Quan [2 ]
机构：
[1] Jilin Agr Univ, Coll Life Sci, Changchun 130118, Jilin Province, Peoples R China
[2] Acad Mil Med Sci, Mil Vet Inst, Changchun 130122, Jilin Province, Peoples R China
来源：
RESEARCH IN VETERINARY SCIENCE | 2015年 / 100卷
基金：
中国国家自然科学基金;
关键词：
Toxoplasma gondii; GRA1; GRA7; Diagnostic marker; ELISA; Chickens; IMMUNOGLOBULIN-G ANTIBODIES; FREE-RANGE CHICKENS; PROTEIN GRA7; IDENTIFICATION; SENSITIVITY; AVIDITY;
D O I：
10.1016/j.rvsc.2015.04.011
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In the present study, recombinant granule antigen proteins GRA1, GRA7 and Toxoplasma soluble antigens (TSA) were evaluated as potential diagnostic markers for T. gondii infection in chickens by an indirect enzyme-linked immunosorbent assay (ELISA), showing a seroprevalence of 16.4% by GRA1-ELISA, and 15.5% by both GRA7- and TSA-ELISA in chickens. No significant difference was observed in the inconsistent results (P > 0.05), and a substantial agreement was found in the consistent results of the 3 tests (92.7-97.3%). Compared with the reference test Western blot, GRA7-ELISA showed the highest copositivity and co-negativity rates. Receiver operating characteristic (ROC) analysis revealed a largest area under curve (AUC) of 0.99 (95% CI, 98-1.0), and a highest relative sensitivity (100%) and specificity (97.9%) for a cut-off value of 0.34 in GRA7-ELISA. These results of the present study showed that GRA7 is a potential diagnostic marker for the detection of 7: gondii infection in chickens. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：161 / 164
页数：4
相关论文
共 28 条
[1]  
Ahn Hye-Jin, 2006, Korean Journal of Parasitology, V44, P303, DOI 10.3347/kjp.2006.44.4.303
[2]   Toxoplasma GRA7 effector increases turnover of immunity-related GTPases and contributes to acute virulence in the mouse [J].
Alaganan, Aditi ;
Fentress, Sarah J. ;
Tang, Keliang ;
Wang, Qiuling ;
Sibley, L. David .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (03) :1126-1131
[3]   EVALUATION OF AN INDIRECT ELISA USING RECOMBINANT GRANULE ANTIGEN GRA7 FOR SERODIAGNOSIS OF TOXOPLASMA GONDII INFECTION IN CATS [J].
Cai, Yufeng ;
Wang, Zedong ;
Li, Jiping ;
Li, Nan ;
Wei, Feng ;
Liu, Quan .
JOURNAL OF PARASITOLOGY, 2015, 101 (01) :37-40
[4]   Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens [J].
Casartelli-Alves, L. ;
Boechat, V. C. ;
Macedo-Couto, R. ;
Ferreira, L. C. ;
Nicolau, J. L. ;
Neves, L. B. ;
Millar, P. R. ;
Vicente, R. T. ;
Oliveira, R. V. C. ;
Muniz, A. G. ;
Bonna, I. C. F. ;
Amendoeira, M. R. R. ;
Silva, R. C. ;
Langoni, H. ;
Schubach, T. M. P. ;
Menezes, R. C. .
VETERINARY PARASITOLOGY, 2014, 204 (3-4) :346-351
[5]   Toxoplasma gondii Infections in Chickens (Gallus domesticus): Prevalence, Clinical Disease, Diagnosis and Public Health Significance [J].
Dubey, J. P. .
ZOONOSES AND PUBLIC HEALTH, 2010, 57 (01) :60-73
[6]   Genetic and biologic characteristics of Toxoplasma gondii isolates in free-range chickens from Colombia, South America [J].
Dubey, JP ;
Gomez-Marin, JE ;
Bedoya, A ;
Lora, F ;
Vianna, MCB ;
Hill, D ;
Kwok, OC ;
Shen, SK ;
Marcet, PL ;
Lehmann, T .
VETERINARY PARASITOLOGY, 2005, 134 (1-2) :67-72
[7]  
DUBEY JP, 1993, AM J VET RES, V54, P1668
[8]   Limited value of assays using detection of immunoglobulin G antibodies to the two recombinant dense granule antigens, GRA1 and GRA6 Nt of Toxoplasma gondii, for distinguishing between acute and chronic infections in pregnant women [J].
Ferrandiz, J ;
Mercier, C ;
Wallon, M ;
Picot, S ;
Cesbron-Delauw, MF ;
Peyron, F .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2004, 11 (06) :1016-1021
[9]  
Hamidinejat H, 2014, TROP BIOMED, V31, P507
[10]   Toxoplasma gondii Recombinant Antigens as Tools for Serodiagnosis of Human Toxoplasmosis: Current Status of Studies [J].
Holec-Gasior, Lucyna .
CLINICAL AND VACCINE IMMUNOLOGY, 2013, 20 (09) :1343-1351
123