In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

被引：41
作者：
He, Junqing [1 ,2 ]
Wang, Junyan [2 ,3 ]
Zhang, Nan [2 ,3 ]
Shen, Luyao [2 ,3 ]
Wang, Linlin [2 ,3 ]
Xiao, Xiao [2 ,3 ]
Wang, Yan [1 ,2 ]
Bing, Tao [2 ,3 ]
Liu, Xiangjun [2 ,3 ]
Li, Songqing [1 ]
Shangguan, Dihua [2 ,3 ]
机构：
[1] Xiangtan Univ, Coll Chem, Xiangtan 411105, Peoples R China
[2] Chinese Acad Sci, Dept Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
TALANTA | 2019年 / 194卷
基金：
中国国家自然科学基金;
关键词：
Aptamer; Cell-SELEX; Ovarian cancer; Drug resistance; Molecular probe; SYSTEMATIC EVOLUTION; MULTIDRUG-RESISTANCE; PROTEIN; GLYCOSYLATION; INHIBITION; LIGANDS; TARGETS; STRESS; CD44; BIND;
D O I：
10.1016/j.talanta.2018.10.028
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Ovarian cancer is regarded as the most lethal gynecologic malignancy with poor prognosis and high mortality rate. Drug-resistance was thought to be the main obstacle to improving overall survival rate of ovarian cancer. New ligands for drug-resistant ovarian cancer cells have potential for the development of diagnosis and therapy of ovarian cancer. In present work, we reported two aptamers, HF3-58 and HA5-68 generated by cell-SELEX, against a paclitaxel-resistant ovarian cancer cell line (A2780T). Both two aptamers exhibited high selectivity and strong affinity to target cells with low nanomolar dissociation constants. The binding of aptamers to target cells was independent of divalent ions, and was tolerant of incubation temperature and nucleases in serum. Molecular targets of the two aptamers were preliminarily demonstrated to be two different glycoproteins on cell surface of A2780T cells. Owing to the structure stability and high resistance to nuclease, these two aptamers had good performance in the detection of drug-resistant ovarian cancer cells in human serum.
引用
收藏
页码：437 / 445
页数：9
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