Macromolecular Crowding in Solutions of Bovine Serum Albumin and Neutral Polymers

被引：0

作者：

Bercea, Maria ^{[1
]}

Plugariu, Ioana-Alexandra ^{[1
]}

机构：

[1] Petru Poni Inst Macromol Chem, 41-A Grigore Ghica Voda Alley, Iasi 700487, Romania

来源：

2021 INTERNATIONAL CONFERENCE ON E-HEALTH AND BIOENGINEERING (EHB 2021), 9TH EDITION | 2021年

关键词：

bovine serum albumin; interaction; pullulan; poly(vinyl alcohol); transient network; phase separation; INTRINSIC VISCOSITIES; MONOCLONAL-ANTIBODY; SELF-ASSOCIATION; AGGREGATION; STABILITY; PROTEINS;

D O I：

10.1109/EHB52898.2021.9657629

中图分类号：

R19 [保健组织与事业（卫生事业管理）];

学科分类号：

摘要：

The paper analyzes the dependence of viscosity on concentration of macromolecules (c) for saline solutions containing bovine serum albumin (BSA), in presence of two water soluble polymers: poly(vinyl alcohol) (PVA) and pullulan (PULL). For dilute solutions, the dependence of relative viscosity on concentration allows the determination of intrinsic viscosity, [eta], through a new model developed by Wolf [Macromolecules, 52, 2019, 3231-3236]. PVA/BSA behaves as an ideal system, whereas intermolecular complexes are formed in the solutions of PULL/BSA. For semidilute and concentrated solutions, the macromolecular crowding influences the overall behavior. New phenomena were observed: a phase separation for PVA/protein concentrated solutions or, extended entangled network mediated by cations present in solution in the case of BSA/PULL mixtures. A generalized behavior describing the viscometric behavior for both systems was obtained from the dependence of the specific viscosity as a function of reduced polymer concentration, cx[eta].

引用

页数：4

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