Cloning, sequencing and expression of the cDNA of endoxylanase B from Penicillium purpurogenum

被引：21
作者：
Diaz, R
Sapag, A
Peirano, A
Steiner, J
Eyzaguirre, J
机构：
[1] PONTIFICIA UNIV CATOLICA CHILE, LAB BIOQUIM, SANTIAGO, CHILE
[2] UNIV CHILE, FAC CIENCIAS QUIM & FARMACEUT, DEPT BIOQUIM & BIOL MOL, SANTIAGO, CHILE
来源：
GENE | 1997年 / 187卷 / 02期
关键词：
endoxylanase; P-purpurogenum; nucleotide sequence; glycosyl hydrolase; PCR; sequence alignment; homology comparison; codon usage;
D O I：
10.1016/S0378-1119(96)00762-7
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The cDNA, for xylanase B from Penicillium purpurogenum was cloned and sequenced. This DNA encodes a protein of 208 amino acids which is expected to yield a protein of 183 residues upon processing of the N terminus. The sequence of the predicted protein is very similar to that of 40 other xylanase domains which belong to family G of cellulases/xylanases (73-21% identity).
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收藏
页码：247 / 251
页数：5
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