Self-assembled extracellular matrix protein networks by microcontact printing

被引：38

作者：

Sgarbi, N ^{[1
]}

Pisignano, D ^{[1
]}

Di Benedetto, F ^{[1
]}

Gigli, G ^{[1
]}

Cingolani, R ^{[1
]}

Rinaldi, R ^{[1
]}

机构：

[1] Univ Lecce, NNL, Ist Nazl Fis Mat, Dipartimento Ingn Innovaz, I-73100 Lecce, Italy

来源：

BIOMATERIALS | 2004年 / 25卷 / 7-8期

关键词：

laminin; micropatterning; protein adsorption; rapid prototyping; atomic force microscopy;

D O I：

10.1016/j.biomaterials.2003.08.017

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Physiological patterns of the extracellular matrix protein, laminin-1, were obtained on glass substrates by physisorption-assisted microcontact printing. Besides the well-retained antigenicity confirmed by-indirect immunofluorescence assays, we investigated the supramolecular organization of the proteins by atomic force microscopy. We found the characteristic protein self-assembling in polygonal networks with well-defined sub-100 nm quaternary structures of laminin. The formation of these physiological mesh-like protein matrices was obtained by means of one-step soft lithography without any preliminary functionalization of glass, which can be exploited for many possible applications for cell cultures and biomolecular devices. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1349 / 1353

页数：5

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