Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

被引：1042

作者：

Matsuyama, Shutoku ^{[1
]}

Nao, Naganori ^{[1
]}

Shirato, Kazuya ^{[1
]}

Kawase, Miyuki ^{[1
]}

Saito, Shinji ^{[2
]}

Takayama, Ikuyo ^{[2
]}

Nagata, Noriyo ^{[3
]}

Sekizuka, Tsuyoshi ^{[4
]}

Katoh, Hiroshi ^{[1
]}

Kato, Fumihiro ^{[1
]}

Sakata, Masafumi ^{[1
]}

Tahara, Maino ^{[1
]}

Kutsuna, Satoshi ^{[5
]}

Ohmagari, Norio ^{[5
]}

Kuroda, Makoto ^{[4
]}

Suzuki, Tadaki ^{[3
]}

Kageyama, Tsutomu ^{[2
]}

Takeda, Makoto ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 3, Tokyo 1628640, Japan

[2] Natl Inst Infect Dis, Influenza Virus Res Ctr, Tokyo 1628640, Japan

[3] Natl Inst Infect Dis, Dept Pathol, Tokyo 1628640, Japan

[4] Natl Inst Infect Dis, Pathogen Genom Ctr, Tokyo 1628640, Japan

[5] Natl Ctr Global Hlth & Med, Dis Control & Prevent Ctr, Tokyo 1628655, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2020年 / 117卷 / 13期

基金：

日本学术振兴会;

关键词：

coronavirus; SARS-CoV-2; VeroE6; outbreak; TMPRSS2; SPIKE PROTEIN; CORONAVIRUS; RECEPTOR;

D O I：

10.1073/pnas.2002589117

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

引用

页码：7001 / 7003

页数：3

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