N6-Methyladenosine RNA Modification Regulates Shoot Stem Cell Fate in Arabidopsis

被引：359

作者：

Shen, Lisha ^{[1
,2
]}

Liang, Zhe ^{[1
,2
]}

Gu, Xiaofeng ^{[3
]}

Chen, Ying ^{[1
,2
]}

Teo, Zhi Wei Norman ^{[1
,2
]}

Hou, Xingliang ^{[4
]}

Cai, Weiling Maggie ^{[5
]}

Dedon, Peter C. ^{[5
]}

Liu, Lu ^{[1
,2
]}

Yu, Hao ^{[1
,2
]}

机构：

[1] Natl Univ Singapore, Temasek Life Sci Lab, 10 Sci Dr 4, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, 10 Sci Dr 4, Singapore 117543, Singapore

[3] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

[4] Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

[5] Singapore MIT Alliance Res & Technol, CREATE, Singapore 138602, Singapore

来源：

DEVELOPMENTAL CELL | 2016年 / 38卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

MESSENGER-RNA; M(6)A RNA; NUCLEAR-RNA; ADENOSINE METHYLATION; GENE; MERISTEM; WUSCHEL; BINDING; N6-METHYLADENOSINE; REPRESSION;

D O I：

10.1016/j.devcel.2016.06.008

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

N-6-Methyladenosine (m(6)A) represents the most prevalent internal modification on mRNA and requires a multicomponent m(6)A methyltransferase complex in mammals. How their plant counterparts determine the global m(6)A modification landscape and its molecular link to plant development remain unknown. Here we show that FKBP12 INTERACTING PROTEIN 37 KD (FIP37) is a core component of the m(6)A methyltransferase complex, which underlies control of shoot stem cell fate in Arabidopsis. The mutants lacking FIP37 exhibit massive overproliferation of shoot meristems and a transcriptome-wide loss of m(6)A RNA modifications. We further demonstrate that FIP37 mediates m(6)A RNA modification on key shoot meristem genes inversely correlated with their mRNA stability, thus confining their transcript levels to prevent shoot meristem overproliferation. Our results suggest an indispensable role of FIP37 in mediating m(6)A mRNA modification, which is required for maintaining the shoot meristem as a renewable source for continuously producing all aerial organs in plants.

引用

页码：186 / 200

页数：15

共 54 条

[1] Immobilization of Proteins in the Nucleolus by Ribosomal Intergenic Spacer Noncoding RNA [J].

Audas, Timothy E. ;

Jacob, Mathieu D. ;

Lee, Stephen .

MOLECULAR CELL, 2012, 45 (02) :147-157

[2]

BARTON MK, 1993, DEVELOPMENT, V119, P823

[3] m6A RNA Modification Controls Cell Fate Transition in Mammalian Embryonic Stem Cells [J].

Batista, Pedro J. ;

Molinie, Benoit ;

Wang, Jinkai ;

Qu, Kun ;

Zhang, Jiajing ;

Li, Lingjie ;

Bouley, Donna M. ;

Lujan, Ernesto ;

Haddad, Bahareh ;

Daneshvar, Kaveh ;

Carter, Ava C. ;

Flynn, Ryan A. ;

Zhou, Chan ;

Lim, Kok-Seong ;

Dedon, Peter ;

Wernig, Marius ;

Mullen, Alan C. ;

Xing, Yi ;

Giallourakis, Cosmas C. ;

Chang, Howard Y. .

CELL STEM CELL, 2014, 15 (06) :707-719

[4] Adenosine methylation in Arabidopsis mRNA is associated with the 3′ end and reduced levels cause developmental defects [J].

Bodi, Zsuzsanna ;

Zhong, Silin ;

Mehra, Surbhi ;

Song, Jie ;

Graham, Neil ;

Li, Hongying ;

May, Sean ;

Fray, Rupert George .

FRONTIERS IN PLANT SCIENCE, 2012, 3

[5] Dependence of stem cell fate in Arabidopsis an a feedback loop regulated by CLV3 activity [J].

Brand, U ;

Fletcher, JC ;

Hobe, M ;

Meyerowitz, EM ;

Simon, R .

SCIENCE, 2000, 289 (5479) :617-619

[6] Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA:: a potential mechanism for the activity of the IME4 gene [J].

Clancy, MJ ;

Shambaugh, ME ;

Timpte, CS ;

Bokar, JA .

NUCLEIC ACIDS RESEARCH, 2002, 30 (20) :4509-4518

[7]

DALMAN FC, 1991, J BIOL CHEM, V266, P3482

[8] Transcriptome-wide mapping of N6-methyladenosine by m6A-seq based on immunocapturing and massively parallel sequencing [J].

Dominissini, Dan ;

Moshitch-Moshkovitz, Sharon ;

Salmon-Divon, Mali ;

Amariglio, Ninette ;

Rechavi, Gideon .

NATURE PROTOCOLS, 2013, 8 (01) :176-189

[9] Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq [J].

Dominissini, Dan ;

Moshitch-Moshkovitz, Sharon ;

Schwartz, Schraga ;

Salmon-Divon, Mali ;

Ungar, Lior ;

Osenberg, Sivan ;

Cesarkas, Karen ;

Jacob-Hirsch, Jasmine ;

Amariglio, Ninette ;

Kupiec, Martin ;

Sorek, Rotem ;

Rechavi, Gideon .

NATURE, 2012, 485 (7397) :201-U84

[10] The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE [J].

Endrizzi, K ;

Moussian, B ;

Haecker, A ;

Levin, JZ ;

Laux, T .

PLANT JOURNAL, 1996, 10 (06) :967-979

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