Immunogenicity and efficacy of the COVID-19 candidate vector vaccine MVA-SARS-2-S in preclinical vaccination

被引：74

作者：

Tscherne, Alina ^{[1
,2
]}

Schwarz, Jan Hendrik ^{[1
]}

Rohde, Cornelius ^{[3
,4
]}

Kupke, Alexandra ^{[3
,4
]}

Kalodimou, Georgia ^{[1
,2
]}

Limpinsel, Leonard ^{[1
]}

Okba, Nisreen M. A. ^{[5
]}

Bosnjak, Berislav ^{[6
]}

Sandrock, Inga ^{[6
]}

Odak, Ivan ^{[6
]}

Halwe, Sandro ^{[3
,4
]}

Sauerhering, Lucie ^{[3
,4
]}

Brosinski, Katrin ^{[1
]}

Nan, Liangliang ^{[1
]}

Duell, Elke ^{[1
,2
]}

Jany, Sylvia ^{[1
]}

Freudenstein, Astrid ^{[1
]}

Schmidt, Joerg ^{[3
,4
]}

Werner, Anke ^{[3
,4
]}

Serra, Michelle Gellhorn ^{[3
,4
]}

Kluever, Michael ^{[3
,4
]}

Guggemos, Wolfgang ^{[7
]}

Seilmaier, Michael ^{[7
]}

Wendtner, Clemens-Martin ^{[7
]}

Foerster, Reinhold ^{[6
,8
,9
]}

Haahmans, Bart L. ^{[5
]}

Becker, Stephan ^{[3
,4
]}

Sutter, Gerd ^{[1
,2
]}

Volz, Asisa ^{[1
,2
,10
]}

机构：

[1] Ludwig Maximilians Univ Munchen, Div Virol, Dept Vet Sci, D-80539 Munich, Germany

[2] German Ctr Infect Res, Dept Vet Sci, Div Virol, D-80539 Munich, Germany

[3] Philipps Univ Marburg, Inst Virol, D-35037 Marburg, Germany

[4] German Ctr Infect Res, Inst Virol, Giessen, Germany

[5] Erasmus MC, Dept Virosci, NL-3015 CN Rotterdam, Netherlands

[6] Hannover Med Sch, Inst Immunol, D-30625 Hannover, Germany

[7] Ludwig Maximilians Univ Munchen, Acad Teaching Hosp, Munich Clin Schwabing, D-80804 Munich, Germany

[8] German Ctr Infect Res, Inst Immunol, D-30625 Hannover, Germany

[9] Hannover Med Sch, Cluster Excellence RESIST, EXC 2155, D-30625 Hannover, Germany

[10] Univ Vet Med, Inst Virol, D-30559 Hannover, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2021年 / 118卷 / 28期

关键词：

vaccine vector; vaccinia virus; poxvirus; nonclinical testing; CORONAVIRUS SPIKE PROTEIN; INFECTION;

D O I：

10.1073/pnas.2026207118

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) has emerged as the infectious agent causing the pandemic coronavi-rus disease 2019 (COVID-19) with dramatic consequences for global human health and economics. Previously, we reached clinical evalua-tion with our vector vaccine based on modified vaccinia virus Ankara (MVA) against the Middle East respiratory syndrome coronavirus (MERS-CoV), which causes an infection in humans similar to SARS and COVID-19. Here, we describe the construction and preclinical char-acterization of a recombinant MVA expressing full-length SARS-CoV-2 spike (S) protein (MVA-SARS-2-S). Genetic stability and growth char-acteristics of MVA-SARS-2-S, plus its robust expression of S protein as antigen, make it a suitable candidate vaccine for industrial-scale pro-duction. Vaccinated mice produced S-specific CD8(+) T cells and serum antibodies binding to S protein that neutralized SARS-CoV-2. Prime -boost vaccination with MVA-SARS-2-S protected mice sensitized with a human ACE2-expressing adenovirus from SARS-CoV-2 infection. MVA-SARS-2-S is currently being investigated in a phase I clinical trial as aspirant for developing a safe and efficacious vaccine against COVID-19.

引用

页数：9

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