Two-dimensional tracking of a motile micro-organism allowing high-resolution observation with various imaging techniques

被引：33

作者：

Oku, H ^{[1
]}

Ogawa, N

Ishikawa, M

Hashimoto, K

机构：

[1] JST PRESTO, Kawaguchi, Saitama 3320012, Japan

[2] Univ Tokyo, Grad Sch Informat Sci & Technol, Tokyo 1138656, Japan

[3] Tohoku Univ, Grad Sch Informat Sci, Sendai, Miyagi 9808579, Japan

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2005年 / 76卷 / 03期

关键词：

Charge coupled devices - Computer vision - Feedback control - High speed cameras - Imaging techniques - Optical microscopy - Photodetectors;

D O I：

10.1063/1.1857632

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

In this article, a micro-organism tracking system using a high-speed vision system is reported. This system two dimensionally tracks a freely swimming micro-organism within the field of an optical microscope by moving a chamber of target micro-organisms based on high-speed visual feedback. The system we developed could track a paramecium using various imaging techniques, including bright-field illumination, dark-field illumination, and differential interference contrast, at magnifications of 5 times and 20 times. A maximum tracking duration of 300 s was demonstrated. Also, the system could track an object with a velocity of up to 35 000 mu m/s (175 diameters/s), which is significantly faster than swimming micro-organisms. (C) 2005 American Institute of Physics.

引用

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