Intraepithelial Lymphocyte Cytometric Pattern Is a Useful Diagnostic Tool for Coeliac Disease Diagnosis Irrespective of Degree of Mucosal Damage and Age-A Validation Cohort

被引：12

作者：

Ruiz-Ramirez, Pablo ^{[1
]}

Carreras, Gerard ^{[1
]}

Fajardo, Ingrid ^{[1
]}

Tristan, Eva ^{[1
,2
]}

Carrasco, Anna ^{[1
,2
]}

Salvador, Isabel ^{[1
]}

Zabana, Yamile ^{[1
,2
]}

Andujar, Xavier ^{[1
,2
]}

Ferrer, Carme ^{[3
]}

Horta, Diana ^{[1
]}

Loras, Carme ^{[1
,2
]}

Garcia-Puig, Roger ^{[4
]}

Fernandez-Banares, Fernando ^{[1
,2
]}

Esteve, Maria ^{[1
,2
]}

机构：

[1] Univ Barcelona, Hosp Univ Mutua Terrassa, Dept Gastroenterol, Barcelona 08221, Spain

[2] Inst Salud Carlos III, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid 28029, Spain

[3] Univ Barcelona, Hosp Univ Mutua Terrassa, Dept Pathol, Barcelona 08221, Spain

[4] Univ Barcelona, Hosp Univ Mutua Terrassa, Dept Pediat, Barcelona 08221, Spain

来源：

NUTRIENTS | 2021年 / 13卷 / 05期

关键词：

coeliac disease; flow cytometry; age; sex; lesion grade; intraepithelial lymphocytes TCR gamma delta(+); FLOW-CYTOMETRY; ANTIBODIES; SENSITIVITY; ETIOLOGY;

D O I：

10.3390/nu13051684

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Introduction: The study of intraepithelial lymphocytes (IEL) by flow cytometry is a useful tool in the diagnosis of coeliac disease (CD). Previous data showed that an increase in %TCR gamma delta(+) and decrease of %CD3(-) IEL constitute a typical CD cytometric pattern with a specificity of 100%. However, there are no data regarding whether there are differences in the %TCR gamma delta(+) related to sex, age, titers of serology, and degree of histological lesion. Study aims: To confirm the high diagnostic accuracy of the coeliac cytometric patterns. To determine if there are differences between sex, age, serology titers, and histological lesion grade. Results: We selected all patients who fulfilled "4 of 5" rule for CD diagnosis (n = 169). There were no differences in %TCR gamma delta(+) between sexes (p = 0.909), age groups (p = 0.986), serology titers (p = 0.53) and histological lesion grades (p = 0.41). The diagnostic accuracy of complete CD cytometric pattern was: specificity 100%, sensitivity 82%, PPV 100%, NPV 47%. Conclusion: We confirmed, in a validation cohort, the high diagnostic accuracy of complete CD pattern irrespective of sex, age, serology titers, and grade of mucosal lesion.

引用

页数：11