Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl.

被引：30
作者：
Tiwari, Jagesh K. [1 ]
Poonam [1 ]
Sarkar, D. [1 ]
Pandey, S. K. [1 ]
Gopal, Jai [1 ]
Kumar, S. Raj [2 ]
机构：
[1] Cent Potato Res Inst, Cell & Mol Biol Lab, Div Crop Improvement, Shimla 171001, Himachal Prades, India
[2] IHBT, Palampur 176061, Himachal Prades, India
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2010年 / 103卷 / 02期
关键词：
Cytoplasm type; Flow cytometry; RAPD; SSR; PVY resistance; POTATO-VIRUS-Y; IMPROVED TOLERANCE; DIHAPLOID CLONES; LEAFROLL-VIRUS; RESISTANCE; DNA; REGENERATION; PROTOPLASTS; INHERITANCE; BERTHAULTII;
D O I：
10.1007/s11240-010-9765-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Interspecific potato somatic hybrids between Solanum tuberosum L. (di)haploid C-13 and 1 endosperm balance number non-tuberous wild species S. etuberosum Lindl. were produced by protoplasts electrofusion. The objective was to transfer virus resistance from this wild species into the cultivated potatoes. Post-fusion products were cultured in VKM medium followed by regeneration of calli in MS13 K medium at 20A degrees C under a 16-h photoperiod, and regenerants were multiplied on MS medium. Twenty-one somatic hybrids were confirmed by RAPD, SSR and cytoplasm (chloroplast/mitochondria) type analysis possessing species-specific diagnostic bands of corresponding parents. Tetraploid nature of these somatic hybrids was determined through flow cytometry analysis. Somatic hybrids showed intermediate phenotypes (plant, leaves and floral morphology) to their parents in glass-house grown plants. All the somatic hybrids were male-fertile. ELISA assay of somatic hybrids after artificial inoculation of Potato virus Y (PVY) infection reveals high PVY resistance.
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页码：175 / 187
页数：13
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