Study of biofilm formation and antibiotic resistance pattern of gram-negative Bacilli among the clinical isolates at BPKIHS, Dharan

被引：51
作者：
Dumaru, Rabina [1 ]
Baral, Ratna [1 ]
Shrestha, Lok Bahadur [1 ]
机构：
[1] BP Koirala Inst Hlth Sci, Microbiol & Infect Dis, Dharan 56700, Nepal
来源：
BMC RESEARCH NOTES | 2019年 / 12卷 / 1期
关键词：
Biofilm; Extended-spectrum beta-lactamases; Gram-negative bacilli; Metallo-beta-lactamases; Multi-drug resistant;
D O I：
10.1186/s13104-019-4084-8
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Objectives: Gram-negative bacilli are the common causative agents for community-acquired, nosocomial and opportunistic infections. The recent upsurge of biofilm, as well as beta-lactamases producing strains, have synergistically led to the extensive dissemination of multi-drug resistant gram-negative bacilli. This study was carried out with an intention to detect the biofilm formation by gram-negative bacilli and determine their antibiogram along with the detection of extended-spectrum beta-lactamases (ESBLs) and metallo-beta-lactamases (MBLs) production. Results: Among 314 isolates, Escherichia coli (38%) were the predominant isolates followed by Acinetobacter spp. (20%), Klebsiella spp. (16%), and Pseudomonas spp. (12%). Overall, 197 (62.73%) of isolates were biofilm positive. 84 (26.75%) and 51 (16.24%) were confirmed as ESBL and MBL producers respectively. The association between MBL production and biofilm formation was statistically significant (chi(2)= 10.20, P value= 0.002) whereas it was insignificant between ESBL and biofilm production (chi(2)= 0.006, P-value= 0.937). Most of the biofilm and MBL producing strains were multi-drug resistant.
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页数：6
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