Degradation of azo dyes by Trametes villosa laccase over long periods of oxidative conditions

被引:124
作者
Zille, A
Górnacka, B
Rehorek, A
Cavaco-Paulo, A
机构
[1] Univ Minho, Dept Text Engn, P-4800058 Guimaraes, Portugal
[2] Univ Appl Sci Cologne, Inst Chem Engn & Plant Sci, D-50679 Cologne, Germany
关键词
D O I
10.1128/AEM.71.11.6711-6718.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulated after 72 h of incubation were analyzed. Liquid chromatography-mass spectrometry (LC-MS) analysis showed the formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerized products that retain azo group integrity. The amino-phenol reactions were also investigated by 13 C-nuclear magnetic resonance and LC-MS analysis, and the polymerization character of laccase was shown. This study highlights the fact that laccases polymerize the reaction products obtained during long-term batch decolorization processes with azo dyes. These polymerized products provide unacceptable color levels in effluents, limiting the application of laccases as bioremediation agents.
引用
收藏
页码:6711 / 6718
页数:8
相关论文
共 36 条
[1]   Kinetics of laccase-catalyzed oxidative polymerization of catechol [J].
Aktas, N ;
Tanyolaç, A .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2003, 22 (1-2) :61-69
[2]  
Anderson JS, 2000, J SOC DYERS COLOUR, V116, P193
[3]   Mechanism of textile metal dye biotransformation by Trametes versicolor [J].
Blánquez, P ;
Casas, N ;
Font, X ;
Gabarrell, X ;
Sarrà, M ;
Caminal, G ;
Vicent, T .
WATER RESEARCH, 2004, 38 (08) :2166-2172
[4]  
BOLLAG JM, 1992, MET IONS BIOL SYST, V28, P205
[5]   Decolorization of azo dyes by Phanerochaete chrysosporium and Pleurotus sajorcaju [J].
Chagas, EP ;
Durrant, LR .
ENZYME AND MICROBIAL TECHNOLOGY, 2001, 29 (8-9) :473-477
[6]  
Chivukula M., 1995, APPL ENVIRON MICROB, V61, P4347
[7]   MUTAGENICITY OF AZO DYES - STRUCTURE-ACTIVITY-RELATIONSHIPS [J].
CHUNG, KT ;
CERNIGLIA, CE .
MUTATION RESEARCH, 1992, 277 (03) :201-220
[8]   DEGRADATION OF AZO DYES BY ENVIRONMENTAL MICROORGANISMS AND HELMINTHS [J].
CHUNG, KT ;
STEVENS, SE .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1993, 12 (11) :2121-2132
[9]   PHOTOOXIDATION OF METHYL-ORANGE SENSITIZED BY ZINC-OXIDE .1. MECHANISM [J].
DARWENT, JR ;
LEPRE, A .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS II, 1986, 82 :1457-+
[10]  
de Hoffman E., 2001, MASS SPECTROMETRY PR