Culture in low levels of oxygen enhances in vitro proliferation potential of satellite cells from old skeletal muscles

被引:78
作者
Chakravarthy, MV
Spangenburg, EE
Booth, FW
机构
[1] Univ Missouri, Dept Vet Biomed Sci, Columbia, MO 65211 USA
[2] Univ Missouri, Dalton Cardiovasc Inst, Columbia, MO 65211 USA
[3] Univ Texas, Sch Med, Dept Integrat Biol, Houston, TX 77054 USA
关键词
hypoxia; stem cell; cellular senescence; aging; cell cycle; Akt;
D O I
10.1007/PL00000929
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The proliferation ability of satellite cells (considered the 'stem cells' of mature myofibers) declines with increasing age when cultured under standard cell culture conditions of 21 % oxygen. However, actual oxygen levels in the intact myofiber in vivo are an order of magnitude lower. No studies to date have addressed the issue of whether culturing satellite cells from old muscles under more 'physiologic' conditions would enhance their proliferation and/or differentiation ability. Therefore, we analyzed satellite cells derived from 31-month-old rats in standard cultures with 21 % O-2 and in lowered (similar to3 %) O-2. Under the lowered O-2 conditions, we noted a remarkable increase in the percentage of large-sized colonies, activation of cell cycle progression factors, phosphorylation of Akt, and downregulation of the cell cycle inhibitor p27(Kip1). These data suggest that lower O-2 levels provide a milieu that stimulates proliferation by allowing continued cell cycle progression, to result ultimately in the enhanced in vitro replicative life span of the old satellite cells. Such a method therefore provides an improved means for the ex vivo generation of progenitor satellite cell populations for potential therapeutic stem cell transplantation.
引用
收藏
页码:1150 / 1158
页数:9
相关论文
共 39 条
[1]   IMMUNOCHEMICAL ANALYSIS OF MYOSIN HEAVY-CHAIN DURING AVIAN MYOGENESIS INVIVO AND INVITRO [J].
BADER, D ;
MASAKI, T ;
FISCHMAN, DA .
JOURNAL OF CELL BIOLOGY, 1982, 95 (03) :763-770
[2]   Viral mediated expression of insulin-like growth factor I blocks the aging-related loss of skeletal muscle function [J].
Barton-Davis, ER ;
Shoturma, DI ;
Musaro, A ;
Rosenthal, N ;
Sweeney, HL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15603-15607
[3]   Hypoxia activates Akt and induces phosphorylation of GSK-3 in PC12 cells [J].
Beitner-Johnson, D ;
Rust, RT ;
Hsieh, TC ;
Millhorn, DE .
CELLULAR SIGNALLING, 2001, 13 (01) :23-27
[4]   EFFECT OF OXYGEN-TENSION ON HEMATOPOIETIC AND FIBROBLAST CELL-PROLIFERATION INVITRO [J].
BRADLEY, TR ;
HODGSON, GS ;
ROSENDAAL, M .
JOURNAL OF CELLULAR PHYSIOLOGY, 1978, 97 (03) :517-522
[5]   Reversible G1 arrest induced by inhibition of the epidermal growth factor receptor tyrosine kinase requires up-regulation of p27KIP1 independent of MAPK activity [J].
Busse, D ;
Doughty, RS ;
Ramsey, TT ;
Russell, WE ;
Price, JO ;
Flanagan, WM ;
Shawver, LK ;
Arteaga, CL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (10) :6987-6995
[6]   Ex vivo expansion of human hematopoietic progenitors and cells to support high-dose chemoradiation therapy: Five years of clinical experience [J].
Chabannon, C ;
Olivero, S ;
Blaise, D ;
Maraninchi, D ;
Viens, P .
CYTOKINES CELLULAR & MOLECULAR THERAPY, 2000, 6 (02) :97-108
[7]   IGF-I restores satellite cell proliferative potential in immobilized old skeletal muscle [J].
Chakravarthy, MV ;
Davis, BS ;
Booth, FW .
JOURNAL OF APPLIED PHYSIOLOGY, 2000, 89 (04) :1365-1379
[8]   Insulin-like growth factor-I extends in vitro replicative life span of skeletal muscle satellite cells by enhancing G1/S cell cycle progression via the activation of phosphatidylinositol 3′-kinase/Akt signaling pathway [J].
Chakravarthy, MV ;
Abraha, TW ;
Schwartz, RJ ;
Fiorotto, ML ;
Booth, FW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (46) :35942-35952
[9]   Inhibition of the phosphoinositide 3-kinase pathway induces a senescence-like arrest mediated by p27Kip1 [J].
Collado, M ;
Medema, RH ;
García-Cao, I ;
Dubuisson, MLN ;
Barradas, M ;
Glassford, J ;
Rivas, C ;
Burgering, BMT ;
Serrano, M ;
Lam, EWF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (29) :21960-21968
[10]   IMMUNOCYTOCHEMICAL ANALYSIS OF INTERMEDIATE FILAMENTS IN EMBRYONIC HEART-CELLS WITH MONOCLONAL-ANTIBODIES TO DESMIN [J].
DANTO, SI ;
FISCHMAN, DA .
JOURNAL OF CELL BIOLOGY, 1984, 98 (06) :2179-2191