Molecular analysis of soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination

被引：12

作者：

Schneider, William L. ^{[1
]}

Damsteegt, Vernon D. ^{[1
]}

Stone, Andrew L. ^{[1
]}

Kuhlmann, Micki ^{[2
]}

Bunyard, Britt A.

Sherman, Diana J. ^{[1
]}

Graves, Michael V. ^{[3
]}

Smythers, Gary ^{[4
]}

Smith, Oney P. ^{[5
]}

Hatziloukas, Efstathios ^{[6
]}

机构：

[1] USDA ARS, FDWSRU, Ft Detrick, MD 21702 USA

[2] Univ Maryland, College Pk, MD 20742 USA

[3] Univ Massachusetts Lowell, Lowell, MA 01854 USA

[4] SAIC Inc Frederick, Nci Frederick, MD 21702 USA

[5] Hood Coll, Frederick, MD 21701 USA

[6] Aristotle Univ Thessaloniki, GR-54006 Thessaloniki, Greece

来源：

VIROLOGY | 2011年 / 412卷 / 01期

关键词：

Soybean dwarf virus; Recombination; Mixed infections; Emerging plant virus; RED-LEAF VIRUS; IDENTIFICATION; DIVERSITY; DISEASES; ROLL;

D O I：

10.1016/j.virol.2011.01.001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Soybean dwarf virus (SbDV), first identified as an agricultural problem in Japan, has emerged as a growing problem in the Midwestern United States. The majority of research on SbDV had been limited to four lab maintained strains from Japan. SbDV had been found in clover in the eastern United States, but these isolates rarely emerged into soybeans. These isolates were analyzed by multiplex PCR and sequencing, revealing that some were infections of both Y and D components, including a recombinant subisolate. Phylogenetic analyses for the US isolates revealed a broad diversity of SbDV, with selection pressure greater on the movement protein than the coat protein. The field isolates from the Eastern United States showed differences in symptoms, aphid transmission and host range, demonstrating that a study of field isolates is an important complement to laboratory maintained strains in understanding the biology and evolution of plant viruses. Published by Elsevier Inc.

引用

页码：46 / 54

页数：9