Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers

被引:158
作者
Luo, Cong [1 ]
He, Xin-Hua [1 ,2 ]
Chen, Hu [1 ]
Ou, Shi-Jin [1 ]
Gao, Mei-Ping [1 ]
机构
[1] Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China
[2] Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China
关键词
SCoT markers; Genetic diversity; Mangifera indica L; MANGIFERA-INDICA L; SEQUENCES;
D O I
10.1016/j.bse.2010.11.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic variation and relationships among 47 mango germplasm and 3 relative species from Guangxi province in China, were analyzed using Start Codon Targeted (SCoT) markers. Using 33 selected SCoT primers 273 bands were generated with an average of 8.27 bands per primer among the 50 accessions, of which 208 (76.19%) were polymorphic. Genetic relationships estimated using the SM similarity coefficient generated values between different pairs of accessions that varied from 0.531 to 0.923 with an average of 0.782. These coefficients were utilized to construct a dendrogram using the UPGMA. All 50 accessions were basically classified into six clusters and correspond well with their recorded pedigrees. The results will provide much more useful information for the management of germplasm and will also be useful to improve the current breeding strategies. The results also demonstrate that the SCoT marker system is useful for identification and genetic diversity analysis of mango cultivars. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1176 / 1184
页数:9
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