miR-200b is involved in intestinal fibrosis of Crohn's disease

被引：93

作者：

Chen, Yingwei ^{[1
,2
,3
]}

Ge, Wensong ^{[2
]}

Xu, Leiming ^{[2
]}

Qu, Chunying ^{[2
]}

Zhu, Mingjie ^{[2
]}

Zhang, Wenzhu ^{[3
]}

Xiao, Yongtao ^{[1
,3
]}

机构：

[1] Shanghai Inst Pediat Res, Shanghai 290092, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Dept Gastroenterol, Xin Hua Hosp, Shanghai 200030, Peoples R China

[3] Shanghai Key Lab Pediat Gastroenterol & Nutr, Shanghai, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2012年 / 29卷 / 04期

关键词：

Crohn's disease; diagnosis; microRNA; epithelial-mesenchymal transition; fibrosis; INFLAMMATORY-BOWEL-DISEASE; CORONARY-ARTERY-DISEASE; N-CADHERIN EXPRESSION; BLOOD-BASED MARKERS; CIRCULATING MICRORNAS; MESENCHYMAL TRANSITION; ALPHA-SMA; MYOCARDIAL-INFARCTION; POTENTIAL BIOMARKERS; CHRONIC HEPATITIS;

D O I：

10.3892/ijmm.2012.894

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Intestinal fibrosis is one of the major serious complications of Crohn's disease (CD). However, there are no effective antifibrotic drugs to treat intestinal fibrosis in CD. Therefore, it is important to understand the pathogenesis of fibrosis in CD. It has been reported that members of the miR-200 family are essential in the regulation of renal fibrogenesis. In this study, we analyzed the function of miR-200a and miR-200b in intestinal fibrosis, which was induced by transforming growth factor beta 1 (TGF-beta 1) in vitro. Furthermore, we detected the expression of miR-200a and miR-200b in CD specimens, which were divided into groups of fibrosis and no-fibrosis. The results of this study showed that administration of miR-200b could partially protect intestinal epithelial cells from fibrogenesis in vitro. Furthermore, we found that miR-200b was overexpressed in the serum of the fibrosis group. The results suggest that miR-200b has potential value for diagnostic and therapeutic applications for CD patients with fibrosis complications.

引用

页码：601 / 606

页数：6

共 41 条

[1] Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction [J].

Ai, Jing ;

Zhang, Rong ;

Li, Yue ;

Pu, Jielin ;

Lu, Yanjie ;

Jiao, Jundong ;

Li, Kang ;

Yu, Bo ;

Li, Zhuqin ;

Wang, Rongrong ;

Wang, Lihong ;

Li, Qiang ;

Wang, Nina ;

Shan, Hongli ;

Li, Zhongyu ;

Yang, Baofeng .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 391 (01) :73-77

[2] The value of α-SMA in the evaluation of hepatic fibrosis severity in hepatitis B infection and cirrhosis development:: a histopathological and immunohistochemical study [J].

Akpolat, N ;

Yahsi, S ;

Godekmerdan, A ;

Yalniz, M ;

Demirbag, K .

HISTOPATHOLOGY, 2005, 47 (03) :276-280

[3] Alpha-SMA expression in hepatic stellate cells and quantitative analysis of hepatic fibrosis in cirrhosis and in recurrent chronic hepatitis after liver transplantation [J].

Carpino, G ;

Morini, S ;

Corradini, SG ;

Franchitto, A ;

Merli, M ;

Siciliano, M ;

Gentili, F ;

Muda, AO ;

Berloco, P ;

Rossi, M ;

Attili, AF ;

Gaudio, E .

DIGESTIVE AND LIVER DISEASE, 2005, 37 (05) :349-356

[4] Circulating MicroRNAs As Potential Biomarkers of Coronary Artery Disease A Promise to Be Fulfilled? [J].

Contu, Riccardo ;

Latronico, Michael V. G. ;

Condorelli, Gianluigi .

CIRCULATION RESEARCH, 2010, 107 (05) :573-574

[5] Circulating microRNAs are new and sensitive biomarkers of myocardial infarction [J].

D'Alessandra, Yuri ;

Devanna, Paolo ;

Limana, Federica ;

Straino, Stefania ;

Di Carlo, Anna ;

Brambilla, Paola G. ;

Rubino, Mara ;

Carena, Maria Cristina ;

Spazzafumo, Liana ;

De Simone, Marco ;

Micheli, Barbara ;

Biglioli, Paolo ;

Achilli, Felice ;

Martelli, Fabio ;

Maggiolini, Stefano ;

Marenzi, Giancarlo ;

Pompilio, Giulio ;

Capogrossi, Maurizio C. .

EUROPEAN HEART JOURNAL, 2010, 31 (22) :2765-2773

[6] Circulating MicroRNAs in Patients With Coronary Artery Disease [J].

Fichtlscherer, Stephan ;

De Rosa, Salvatore ;

Fox, Henrik ;

Schwietz, Thomas ;

Fischer, Ariane ;

Liebetrau, Christoph ;

Weber, Michael ;

Hamm, Christian W. ;

Roexe, Tino ;

Mueller-Ardogan, Marga ;

Bonauer, Angelika ;

Zeiher, Andreas M. ;

Dimmeler, Stefanie .

CIRCULATION RESEARCH, 2010, 107 (05) :677-U257

[7] A switch from E-cadherin to N-cadherin expression indicates epithelial to mesenchymal transition and is of strong and independent importance for the progress of prostate cancer [J].

Gravdal, Karsten ;

Halvorsen, Ole J. ;

Haukaas, Svein A. ;

Akslen, Lars A. .

CLINICAL CANCER RESEARCH, 2007, 13 (23) :7003-7011

[8] The mir-200 family and mir-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 [J].

Gregory, Philip A. ;

Bert, Andrew G. ;

Paterson, Emily L. ;

Barry, Simon C. ;

Tsykin, Anna ;

Farshid, Gelareh ;

Vadas, Mathew A. ;

Khew-Goodall, Yeesim ;

Goodall, Gregory J. .

NATURE CELL BIOLOGY, 2008, 10 (05) :593-601

[9] Circulating MicroRNAs as Biomarkers and Potential Paracrine Mediators of Cardiovascular Disease [J].

Gupta, Shashi K. ;

Bang, Claudia ;

Thum, Thomas .

CIRCULATION-CARDIOVASCULAR GENETICS, 2010, 3 (05) :484-488

[10] Circulating microRNAs as Novel Minimally Invasive Biomarkers for Breast Cancer [J].

Heneghan, Helen M. ;

Miller, Nicola ;

Lowery, Aoife J. ;

Sweeney, Karl J. ;

Newell, John ;

Kerin, Michael J. .

ANNALS OF SURGERY, 2010, 251 (03) :499-505

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