Comparative Profiling Analysis of Central Metabolites in Euglena gracilis under Various Cultivation Conditions

被引：35

作者：

Matsuda, Fumio ^{[2
]}

Hayashi, Masahiro ^{[3
]}

Kondo, Akihiko ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Engn, Dept Sci & Chem Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

[3] Miyazaki Univ, Fac Agr, Dept Marine Biol & Environm Sci, Miyazaki 8892192, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2011年 / 75卷 / 11期

关键词：

Euglena gracilis; metabolic profiling analysis; wax ester; fermentation; CHROMATOGRAPHY-MASS SPECTROMETRY; CYTOSOLIC PYRUVATE-KINASE; WAX ESTERS; BETA-1,3-GLUCAN; GLYCOLYSIS; BREAKDOWN; PATHWAY; GROWTH; ENERGY; CARBON;

D O I：

10.1271/bbb.110482

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative metabolic profiling analysis was performed to investigate light- and aeration-dependent regulation of central metabolism in Englena gracilis. The metabolic profiles of E. gracilis were significantly altered in response to changes in aeration conditions. While many glycolytic intermediates and amino acids accumulated in aerobically grown E. gracilis, a significant reduction in these metabolites was observed for cells under anaerobic conditions, which resulted in elevated production of wax ester.

引用

页码：2253 / 2256

页数：4

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