Estimating the dual-enzyme kinetic parameters for Cr (VI) reduction by Shewanella oneidensis MR-1 from soil column experiments

被引:9
作者
Hossain, A [1 ]
Alam, M
Yonge, DR
机构
[1] Washington State Univ, Richland, WA 99354 USA
[2] Washington State Univ, Pullman, WA 99164 USA
关键词
dual-enzyme; parameters; Cr (VI); bioreduction; column;
D O I
10.1016/j.watres.2005.05.033
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Hexavalent chromium (Cr (VI)) contamination of soil and groundwater is considered a major environmental concern. Bioreduction of Cr (VI) to trivalent chromium (Cr (III)) can be considered an effective technology in remediating Cr (VI) contaminated sites. Among the Cr (VI) reducing bacteria, Shewanella oneidensis MR-1 (MR-1) is relatively effective. Reduction of Cr (VI) by MR-1 is defined by the dual-enzyme kinetic model. The feasibility of bioreduction of Cr (VI) is usually evaluated by performing batch experiments which may not accurately represent the subsurface environment. The objective of this paper is to present a rational approach to determine the dual-enzyme reaction kinetics of Cr (VI) reduction by MR-1 from continuous flow soil column experiments which more accurately simulate the subsurface environment. Kinetic parameters obtained from soil column data were found to be much smaller than that obtained from batch data. The stable enzyme induced reaction rate was found to be 1/27th and the average deactivating enzyme induced reaction rate was 1/17th of the corresponding values obtained from batch reactor data as reported in literature. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3342 / 3348
页数:7
相关论文
共 26 条
[1]  
Barnhart J, 1997, J SOIL CONTAM, V6, P561, DOI 10.1080/15320389709383589
[2]   CHROMATE RESISTANCE AND REDUCTION IN PSEUDOMONAS-FLUORESCENS STRAIN LB300 [J].
BOPP, LH ;
EHRLICH, HL .
ARCHIVES OF MICROBIOLOGY, 1988, 150 (05) :426-431
[3]   Environmental factors and modeling in microbial chromium (VI) reduction [J].
Chen, JM ;
Hao, OJ .
WATER ENVIRONMENT RESEARCH, 1996, 68 (07) :1156-1164
[4]  
Chen JM, 1997, J CHEM TECHNOL BIOT, V69, P70, DOI 10.1002/(SICI)1097-4660(199705)69:1<70::AID-JCTB665>3.0.CO
[5]  
2-4
[6]  
Davis John H, 1993, METALS GROUNDWATER, P223
[7]  
DRAGUN J, 1988, ELEMENT FIXATION SOI, P75
[8]   DEVELOPMENT OF A BIOREACTOR SYSTEM FOR THE TREATMENT OF CHROMATE WASTE-WATER USING ENTEROBACTER-CLOACAE HO1 [J].
FUJIE, K ;
TSUCHIDA, T ;
URANO, K ;
OHTAKE, H .
WATER SCIENCE AND TECHNOLOGY, 1994, 30 (03) :235-243
[9]  
FUJIE K, 1996, WATER SCI TECHNOL, V43, P173
[10]  
HORITSU H, 1987, AGR BIOL CHEM TOKYO, V51, P2417