Cloning and sequencing of the secY homolog from Streptomyces lividans 1326

被引:7
|
作者
Ostiguy, S
Gilbert, M
Shareck, F
Kluepfel, D
Morosoli, R
机构
[1] UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH MICROBIOL APPL, LAVAL, PQ H7N 4Z3, CANADA
[2] NATL RES COUNCIL CANADA, INST BIOL SCI, OTTAWA, ON K1A 0R6, CANADA
关键词
actinomycetes; protein translocation; secretion; operon; ribosomal protein L15; adenylate kinase;
D O I
10.1016/0378-1119(96)00229-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Two conserved regions of SecY proteins from six Gram(+) bacteria were exploited in a PCR-based strategy for isolating a secY homolog from Streptomyces lividans (Sl). The nucleotide sequence of part of a 3.8-kb fragment showed that the secY homolog is flanked, at the 5' end, by the gene encoding ribosomal protein L15 and, at the 3' end, by an adenylate kinase-encoding gene. The deduced gene product of secY would have 437 amino acids (aa) and an M(r) of 47 200. Sl SecY shows 89.5, 56.1, 42 and 40% identity to its homologs from Streptomyces scabies, Brevibacterium flavum, Bacillus subtilis and Escherichia coli, respectively. Promoter-probe analyses indicated that the secY gene probably contains its own promoter.
引用
收藏
页码:265 / 267
页数:3
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